TTK siRNA (Human)
Cat# MBS829007-15nmol
Size : 15nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
TTK siRNA – Dual Specificity Protein Kinase TTK Isoform 2 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829007MBS829007 is an siRNA targeting TTK. Also known as TTK siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit TTK expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
TTK, siRNA
Full Product Name
TTK siRNA (Human)
Product Gene Name
TTK sirna
Product Synonym Names
MPS1; MPS1L1; Dual specificity protein kinase TTK; Phosphotyrosine picked threonine-protein kinase; PYT
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
TTK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
96,944 Da
OMIM
604092
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TTK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for TTK sirna
RNA Interference (RNAi)
Other Notes
Small volumes of TTK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TTK. It may not necessarily be applicable to this product.
NCBI Official Full Name
dual specificity protein kinase TTK isoform 2
NCBI Official Synonym Full Names
TTK protein kinase
NCBI Official Symbol
TTK
NCBI Official Synonym Symbols
ESK; PYT; CT96; MPH1; MPS1; MPS1L1
UniProt Protein Name
Dual specificity protein kinase TTK
UniProt Synonym Protein Names
Phosphotyrosine picked threonine-protein kinase; PYT
Protein Family
Dual specificity protein kinase
UniProt Gene Name
TTK
UniProt Synonym Gene Names
MPS1; MPS1L1; PYT
UniProt Entry Name
TTK_HUMAN
NCBI Protein Information
dual specificity protein kinase TTK
NCBI Summary for TTK
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
Show more UniProt Comments for TTK
TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.
Protein type: Protein kinase, dual-specificity (non-receptor); Protein kinase, Other; Cancer Testis Antigen (CTA); EC 2.7.12.1; Kinase, protein; Other group; TTK family
Chromosomal Location of Human Ortholog: 6q14.1
Cellular Component: membrane; spindle
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding
Biological Process: mitotic spindle organization and biogenesis; peptidyl-tyrosine phosphorylation; mitotic cell cycle spindle assembly checkpoint; positive regulation of cell proliferation; spindle organization and biogenesis; chromosome separation
Show more Protein type: Protein kinase, dual-specificity (non-receptor); Protein kinase, Other; Cancer Testis Antigen (CTA); EC 2.7.12.1; Kinase, protein; Other group; TTK family
Chromosomal Location of Human Ortholog: 6q14.1
Cellular Component: membrane; spindle
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding
Biological Process: mitotic spindle organization and biogenesis; peptidyl-tyrosine phosphorylation; mitotic cell cycle spindle assembly checkpoint; positive regulation of cell proliferation; spindle organization and biogenesis; chromosome separation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TTK sirna
| Reference Product | PubMed Publications |
|---|---|
| BUB1 sirna | >24 publications with TTK and BUB1 |
| MAD2L1 sirna | >19 publications with TTK and MAD2L1 |
| MAD1L1 sirna | >12 publications with TTK and MAD1L1 |
| CDC20 sirna | >9 publications with TTK and CDC20 |
| NDC80 sirna | >9 publications with TTK and NDC80 |
| CDK2 sirna | >8 publications with TTK and CDK2 |
| NUF2 sirna | >5 publications with TTK and NUF2 |
| CDC27 sirna | >1 publications with TTK and CDC27 |
Pathways associated with TTK sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| RB In Cancer Pathway antibodies | RB In Cancer Pathway Diagram |
Diseases associated with TTK sirna
| Disease Name | Pubmed Publications |
|---|---|
| Brain Diseases Antibodies | >19 publications with TTK and Brain Diseases |
| Breast Neoplasms Antibodies | >16 publications with TTK and Breast Neoplasms |
| Disease Models, Animal Antibodies | >12 publications with TTK and Disease Models, Animal |
| Lung Neoplasms Antibodies | >8 publications with TTK and Lung Neoplasms |
| Carcinoma, Hepatocellular Antibodies | >8 publications with TTK and Carcinoma, Hepatocellular |
| Neoplasms, Experimental Antibodies | >7 publications with TTK and Neoplasms, Experimental |
| Lung Diseases Antibodies | >7 publications with TTK and Lung Diseases |
| Liver Diseases Antibodies | >6 publications with TTK and Liver Diseases |
| Cell Transformation, Neoplastic Antibodies | >6 publications with TTK and Cell Transformation, Neoplastic |
| Liver Neoplasms Antibodies | >5 publications with TTK and Liver Neoplasms |
Organs/Tissues associated with TTK sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >58 publications with TTK and Brain |
| Eye Antibodies | >40 publications with TTK and Eye |
| Blood Antibodies | >38 publications with TTK and Blood |
| Muscle Antibodies | >17 publications with TTK and Muscle |
| Heart Antibodies | >15 publications with TTK and Heart |
| Embryonic Tissue Antibodies | >13 publications with TTK and Embryonic Tissue |
| Liver Antibodies | >12 publications with TTK and Liver |
| Testis Antibodies | >11 publications with TTK and Testis |
| Bone Antibodies | >9 publications with TTK and Bone |
| Vascular Antibodies | >8 publications with TTK and Vascular |