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ABO siRNA (Rat)

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Cat# MBS826936-15nmol

Size : 15nmol

Brand : MyBiosource

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Phone : +1 850 650 7790

ABO siRNA – Histo-Blood Group ABO System Transferase 1 (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS826936

MBS826936 is an siRNA targeting ABO. Also known as ABO siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit ABO expression using RNA interference More details

Product Overview

Online Datasheet in PDF
Product Name
ABO, siRNA
Full Product Name
ABO siRNA (Rat)
Product Gene Name
ABO sirna
Product Synonym Names
ABO1; Histo-blood group ABO system transferase 1; Blood group A glycosyltransferase 1; Cis-AB transferase 1; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Histo-blood group A transferase; A transferase; Histo-blood group B transferase; B transferase; N-acetylgalactosaminyltransferase A blood group-like enzyme; NAGAT 1
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ABO siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
40,375 Da
3D Structure
ModBase 3D Structure for Q9ET32
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ABO gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ABO sirna
RNA Interference (RNAi)

Other Notes

Small volumes of ABO sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ABO. It may not necessarily be applicable to this product.

NCBI GI #
148747204
NCBI GeneID
65270
NCBI Accession #
NP_075582.3
UniProt Primary Accession #
Q9ET32
UniProt Secondary Accession #
Q8CFC5; Q8CFC6; Q8R005; Q8R4Y3; Q8R552
UniProt Related Accession #
Q9ET32
GenBank Nucleotide #
NM_023094.3
NCBI Official Full Name
histo-blood group ABO system transferase 1
NCBI Official Synonym Full Names
globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
NCBI Official Symbol
Gbgt1
NCBI Official Synonym Symbols
Abo; Abol1
UniProt Protein Name
Histo-blood group ABO system transferase 1
UniProt Synonym Protein Names
Blood group A glycosyltransferase 1; Cis-AB transferase 1; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40); Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37); Histo-blood group A transferase; A transferase; Histo-blood group B transferase; B transferase; N-acetylgalactosaminyltransferase A blood group-like enzyme; NAGAT 1
Protein Family
4-methylaminobutanoate oxidase
UniProt Gene Name
Abo
UniProt Synonym Gene Names
Abo1; A transferase; B transferase
UniProt Entry Name
BGAT1_RAT
NCBI Protein Information
histo-blood group ABO system transferase 1

NCBI Summary for ABO

histo-blood group protein with A- and B-transferase activities [RGD, Feb 2006]

UniProt Comments for ABO

Posseses strong A transferase activity and a weak B transferase activity.

Research Articles on ABO

1. data indicate that rats have at least two distinct Abo genes, one monomorphic gene encoding a B-specific enzyme and one or more genes in some cases encoding an A-specific enzyme

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ABO sirna

 Reference Product  PubMed Publications
 Abo sirna  >2 publications with ABO and Abo

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