Active Colony Stimulating Factor 1, Macrophage (MCSF)
Cat# MBS2097263-2mg
Size : 2mg
Brand : MyBiosource
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Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.
Colony Stimulating Factor 1, Macrophage (MCSF) Mouse Active Protein
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2097263SPECIAL PROMOTION: Get FREE Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
MBS2097263 is an active protein targeting MCSF. Also known as Active Colony Stimulating Factor 1, Macrophage (MCSF). Reactive in Mouse. Host: E Coli. More details
Product Overview
Online Datasheet in PDFProduct Name
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein
Full Product Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Product Gene Name
MCSF active protein
Product Synonym Names
CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1
Host
E Coli
Species Reactivity
Mouse
Form/Format
Freeze-dried powder
Purity/Purification
> 95%
Molecular Weight
Predicted Molecular Weight: 23.5kDa
Accurate Molecular Weight: 24kDa
Accurate Molecular Weight: 24kDa
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Lys33~Ser204
Isoelectric Point
6
Product Categories/Family for MCSF active protein
Cytokine; Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for MCSF active protein
Cell culture, Activity Assays
Activity Data of MCSF active protein
Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.
Other Notes
Small volumes of MCSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for MCSF. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
macrophage colony-stimulating factor 1 isoform 2
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
Csf1
NCBI Official Synonym Symbols
op; Csfm; MCSF; C87615
UniProt Protein Name
Macrophage colony-stimulating factor 1
UniProt Gene Name
Csf1
UniProt Synonym Gene Names
Csfm; CSF-1; MCSF
NCBI Protein Information
macrophage colony-stimulating factor 1
UniProt Comments for MCSF
M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3 F2.3|3 46.83 cM
Cellular Component: extracellular region; extracellular space; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein binding; protein homodimerization activity
Biological Process: cell proliferation; homeostasis of number of cells within a tissue; immune system process; inflammatory response; innate immune response; macrophage differentiation; odontogenesis; ossification; osteoclast differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of mononuclear cell proliferation; positive regulation of multicellular organism growth; positive regulation of odontogenesis of dentin-containing tooth; positive regulation of osteoclast differentiation; positive regulation of protein kinase activity; positive regulation of Ras protein signal transduction; regulation of ossification; reproductive developmental process; transmembrane receptor protein tyrosine kinase signaling pathway
Show more Protein type: Cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3 F2.3|3 46.83 cM
Cellular Component: extracellular region; extracellular space; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein binding; protein homodimerization activity
Biological Process: cell proliferation; homeostasis of number of cells within a tissue; immune system process; inflammatory response; innate immune response; macrophage differentiation; odontogenesis; ossification; osteoclast differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of mononuclear cell proliferation; positive regulation of multicellular organism growth; positive regulation of odontogenesis of dentin-containing tooth; positive regulation of osteoclast differentiation; positive regulation of protein kinase activity; positive regulation of Ras protein signal transduction; regulation of ossification; reproductive developmental process; transmembrane receptor protein tyrosine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with MCSF active protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokines And Inflammatory Response (BioCarta) Pathway antibodies | Cytokines And Inflammatory Response (BioCarta) Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Osteoclast Differentiation Pathway antibodies | Osteoclast Differentiation Pathway Diagram |
| Osteoclast Differentiation Pathway antibodies | Osteoclast Differentiation Pathway Diagram |
| PI3K-Akt Signaling Pathway antibodies | PI3K-Akt Signaling Pathway Diagram |
| PI3K-Akt Signaling Pathway antibodies | PI3K-Akt Signaling Pathway Diagram |
| Rap1 Signaling Pathway antibodies | Rap1 Signaling Pathway Diagram |
Diseases associated with MCSF active protein
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >38 publications with MCSF and Inflammation |
| Carcinoma Antibodies | >26 publications with MCSF and Carcinoma |
| Necrosis Antibodies | >21 publications with MCSF and Necrosis |
| Breast Neoplasms Antibodies | >21 publications with MCSF and Breast Neoplasms |
| Adenocarcinoma Antibodies | >14 publications with MCSF and Adenocarcinoma |
| Lung Neoplasms Antibodies | >14 publications with MCSF and Lung Neoplasms |
| Neoplasms, Experimental Antibodies | >10 publications with MCSF and Neoplasms, Experimental |
| Lung Diseases Antibodies | >9 publications with MCSF and Lung Diseases |
| Liver Diseases Antibodies | >7 publications with MCSF and Liver Diseases |
| Osteitis Deformans Antibodies | >6 publications with MCSF and Osteitis Deformans |
Organs/Tissues associated with MCSF active protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >70 publications with MCSF and Blood |
| Bone Antibodies | >61 publications with MCSF and Bone |
| Bone Marrow Antibodies | >34 publications with MCSF and Bone Marrow |
| Lung Antibodies | >28 publications with MCSF and Lung |
| Brain Antibodies | >16 publications with MCSF and Brain |
| Connective Tissue Antibodies | >13 publications with MCSF and Connective Tissue |
| Lymph Node Antibodies | >12 publications with MCSF and Lymph Node |
| Kidney Antibodies | >10 publications with MCSF and Kidney |
| Intestine Antibodies | >10 publications with MCSF and Intestine |
| Spleen Antibodies | >9 publications with MCSF and Spleen |