Active Colony Stimulating Factor 1, Macrophage (MCSF)
Cat# MBS2162198-0,1mg
Size : 0.1mg
Brand : MyBiosource
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Figure. SDS-PAGE
Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human M-CSF and recombinant human Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra). Briefly, biotin-linked M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human M-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.
Colony Stimulating Factor 1, Macrophage (MCSF) Active Protein
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2162198SPECIAL PROMOTION: Get FREE Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
MBS2162198 is an active protein targeting MCSF. Also known as Active Colony Stimulating Factor 1, Macrophage (MCSF). Host: E Coli. More details
Product Overview
Online Datasheet in PDFProduct Name
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein
Full Product Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Product Gene Name
MCSF active protein
Product Synonym Names
Colony Stimulating Factor 1, Macrophage; CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 14
Sequence
Glu33~Asn1 90
Host
E Coli
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Purity/Purification
Greater than 95% by SDS-PAGE
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Molecular Weight
60,179 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (110453233..110473616). Location: 1p13.3
OMIM
120420
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
4.6
Research Area
Cytokine, Infection immunity
Product Categories/Family for MCSF active protein
Active proteins
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for MCSF active protein
Cell culture, Activity Assays
Activity of MCSF active protein
Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human M-CSF and recombinant human Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra). Briefly, biotin-linked M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human M-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.
Other Notes
Small volumes of MCSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for MCSF. It may not necessarily be applicable to this product.
NCBI Official Full Name
colony-stimulating factor (CSF-1)
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
CSF1
NCBI Official Synonym Symbols
MCSF; CSF-1
UniProt Protein Name
Macrophage colony-stimulating factor 1
UniProt Synonym Protein Names
Lanimostim
UniProt Gene Name
CSF1
UniProt Synonym Gene Names
CSF-1; M-CSF; MCSF
UniProt Entry Name
CSF1_HUMAN
NCBI Protein Information
macrophage colony-stimulating factor 1; lanimostim
NCBI Summary for MCSF
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
Show more UniProt Comments for MCSF
M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cytokine
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; receptor complex
Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding
Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; monocyte activation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; hemopoiesis; positive regulation of Ras protein signal transduction; positive regulation of macrophage differentiation; cell differentiation; positive regulation of cell-matrix adhesion; inflammatory response; regulation of ossification; positive regulation of cell migration
Show more Protein type: Membrane protein, integral; Cytokine
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; receptor complex
Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding
Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; monocyte activation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; hemopoiesis; positive regulation of Ras protein signal transduction; positive regulation of macrophage differentiation; cell differentiation; positive regulation of cell-matrix adhesion; inflammatory response; regulation of ossification; positive regulation of cell migration
Research Articles on MCSF
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with MCSF active protein
| Reference Product | PubMed Publications |
|---|---|
| CSF1R active protein | >67 publications with MCSF and CSF1R |
| JUN active protein | >19 publications with MCSF and JUN |
| GAPDH active protein | >3 publications with MCSF and GAPDH |
| MYC active protein | >3 publications with MCSF and MYC |
| ITGB3 active protein | >1 publications with MCSF and ITGB3 |
Pathways associated with MCSF active protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokines And Inflammatory Response Pathway antibodies | Cytokines And Inflammatory Response Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Integrins In Angiogenesis Pathway antibodies | Integrins In Angiogenesis Pathway Diagram |
| Osteoclast Differentiation Pathway antibodies | Osteoclast Differentiation Pathway Diagram |
| Osteoclast Differentiation Pathway antibodies | Osteoclast Differentiation Pathway Diagram |
| PI3K-Akt Signaling Pathway antibodies | PI3K-Akt Signaling Pathway Diagram |
| PI3K-Akt Signaling Pathway antibodies | PI3K-Akt Signaling Pathway Diagram |
Diseases associated with MCSF active protein
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >59 publications with MCSF and Neoplasms |
| Inflammation Antibodies | >25 publications with MCSF and Inflammation |
| Carcinoma Antibodies | >20 publications with MCSF and Carcinoma |
| Breast Neoplasms Antibodies | >14 publications with MCSF and Breast Neoplasms |
| Necrosis Antibodies | >13 publications with MCSF and Necrosis |
| Lung Neoplasms Antibodies | >9 publications with MCSF and Lung Neoplasms |
| Liver Diseases Antibodies | >7 publications with MCSF and Liver Diseases |
| Neoplasms, Experimental Antibodies | >6 publications with MCSF and Neoplasms, Experimental |
| Osteitis Deformans Antibodies | >6 publications with MCSF and Osteitis Deformans |
| Lung Diseases Antibodies | >5 publications with MCSF and Lung Diseases |
Organs/Tissues associated with MCSF active protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >53 publications with MCSF and Blood |
| Bone Antibodies | >50 publications with MCSF and Bone |
| Lung Antibodies | >20 publications with MCSF and Lung |
| Liver Antibodies | >15 publications with MCSF and Liver |
| Brain Antibodies | >13 publications with MCSF and Brain |
| Connective Tissue Antibodies | >9 publications with MCSF and Connective Tissue |
| Intestine Antibodies | >7 publications with MCSF and Intestine |
| Kidney Antibodies | >7 publications with MCSF and Kidney |
| Nerve Antibodies | >6 publications with MCSF and Nerve |
| Spleen Antibodies | >6 publications with MCSF and Spleen |