Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1)
Cat# MBS8005532-2x0,1mg
Size : 2x0.1mg
Brand : MyBiosource
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TEM of A53T alpha synuclein preformed fibrils (MBS8005532)
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers ( MBS8005531 ). A much greater increase in fluorescence is seen when 100 uM monomer (MBS8005531) is combined with 10 nM of fibrils (MBS8005532) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.
A53T Mutant Alpha Synuclein (A53T) Human Recombinant Active Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8005532MBS8005532 is an active protein targeting A53T. Also known as Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1). Host: E Coli. More details
Product Overview
Online Datasheet in PDFProduct Name
A53T Mutant Alpha Synuclein, Active Protein
Full Product Name
Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1)
Product Gene Name
A53T active protein
Product Synonym Names
Alpha Synuclein Protein; A53T mutant alpha synuclein; A53T mutated SNCA; A53T Alpha synuclein PFFs; Ala-53-->thr mutant alpha synuclein; Alpha synuclein pre-formed fibrils; Alpha synuclein aggregates; Alpha synuclein protein aggregates; Active Alpha synuclein aggregates; Alpha-synuclein protein; Non-A beta component of AD amyloid protein; Non-A4 component of amyloid precursor protein; NACP protein; SNCA protein; NACP protein; PARK1 protein; Alpha synuclein monomers; SYN protein; Parkinson disease familial 1 Protein
Host
E Coli
Form/Format
PBS
Purity/Purification
>95% pure using SDS-PAGE analysis.
Ion-Exchange Purified
Ion-Exchange Purified
Concentration
2 mg/mL (lot specific)
Specificity
~14.46 kDa
Preparation and Storage
Store at-80°C for 1 year. Avoid freeze/thaw cycle. Ships on Dry Ice.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Nature
Recombinant
Cellular Localization
Cytoplasm, Membrane, Nucleus
Protein Length
Full Length
Biological Activity
Endogenous alpha-synuclein phosphorylation. 100 uM alpha synuclein protein monomer (MBS8001845) seeded with 10 nM alpha synuclein protein aggregatein 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Research Areas
Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Tangles & Tau
Function
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease (7) and increased rates of alpha synuclein fibrillization (8).
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Categories/Family for A53T active protein
Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein
Applications Tested/Suitable for A53T active protein
Western Blot (WB), SDS-PAGE, In Vitro Assay, In Vivo Assay
Testing Data of A53T active protein
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers ( MBS8005531 ). A much greater increase in fluorescence is seen when 100 uM monomer (MBS8005531) is combined with 10 nM of fibrils (MBS8005532) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.
Product References and Citations for A53T active protein
1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Polymeropoulos, M. H. (1998). Science. 276(5321), 2045-2047. 8. Conway, K.E., et al. (1998). Nat Med. 4(11):1318-20.
Other Notes
Small volumes of A53T active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for A53T. It may not necessarily be applicable to this product.
NCBI Official Full Name
alpha-synuclein isoform NACP140
NCBI Official Synonym Full Names
synuclein alpha
NCBI Official Symbol
SNCA
NCBI Official Synonym Symbols
PD1; NACP; PARK1; PARK4
NCBI Protein Information
alpha-synuclein
NCBI Summary for A53T
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with A53T active protein
| Reference Product | PubMed Publications |
|---|---|
| SNCB active protein | >74 publications with A53T and SNCB |
| MAPT active protein | >64 publications with A53T and MAPT |
| SNCAIP active protein | >31 publications with A53T and SNCAIP |
| PARK2 active protein | >30 publications with A53T and PARK2 |
| APP active protein | >16 publications with A53T and APP |
| SLC6A3 active protein | >12 publications with A53T and SLC6A3 |
| MAPK1 active protein | >5 publications with A53T and MAPK1 |
| TOR1A active protein | >4 publications with A53T and TOR1A |
| BAD active protein | >3 publications with A53T and BAD |
| SQSTM1 active protein | >3 publications with A53T and SQSTM1 |
Pathways associated with A53T active protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Alpha-synuclein Signaling Pathway antibodies | Alpha-synuclein Signaling Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimers Disease Pathway antibodies | Alzheimers Disease Pathway Diagram |
| Amyloids Pathway antibodies | Amyloids Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| EGFR1 Signaling Pathway antibodies | EGFR1 Signaling Pathway Diagram |
| Parkin-Ubiquitin Proteasomal System Pathway antibodies | Parkin-Ubiquitin Proteasomal System Pathway Diagram |
| Parkinson's Disease Pathway antibodies | Parkinson's Disease Pathway Diagram |
| Parkinsons Disease Pathway antibodies | Parkinsons Disease Pathway Diagram |
Diseases associated with A53T active protein
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >1049 publications with A53T and Nervous System Diseases |
| Brain Diseases Antibodies | >1028 publications with A53T and Brain Diseases |
| Neurodegenerative Diseases Antibodies | >1016 publications with A53T and Neurodegenerative Diseases |
| Movement Disorders Antibodies | >896 publications with A53T and Movement Disorders |
| Parkinsonian Disorders Antibodies | >829 publications with A53T and Parkinsonian Disorders |
| Parkinson Disease Antibodies | >755 publications with A53T and Parkinson Disease |
| Lewy Body Disease Antibodies | >270 publications with A53T and Lewy Body Disease |
| Nerve Degeneration Antibodies | >160 publications with A53T and Nerve Degeneration |
| Disease Models, Animal Antibodies | >127 publications with A53T and Disease Models, Animal |
| Drug Toxicity Antibodies | >71 publications with A53T and Drug Toxicity |
Organs/Tissues associated with A53T active protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Nerve Antibodies | >986 publications with A53T and Nerve |
| Brain Antibodies | >718 publications with A53T and Brain |
| Blood Antibodies | >53 publications with A53T and Blood |
| Embryonic Tissue Antibodies | >28 publications with A53T and Embryonic Tissue |
| Kidney Antibodies | >19 publications with A53T and Kidney |
| Heart Antibodies | >9 publications with A53T and Heart |
| Eye Antibodies | >8 publications with A53T and Eye |
| Skin Antibodies | >6 publications with A53T and Skin |
| Bone Antibodies | >6 publications with A53T and Bone |
| Liver Antibodies | >6 publications with A53T and Liver |