Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Adiponectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Adiponectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Adiponectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Adiponectin can be calculated.

Background/Introduction: Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene. This gene spans 16 kb, contains 3 exons, and maps to to chromosome 3q27. Adiponectin is a 244-amino-acid-long polypeptide, and it has four distinct regions. It is a hormone secreted by adipocytes (and also from the placenta in pregnancy) that regulates energy homeostasis and glucose and lipid metabolism. Adipocytes also produce and secrete proteins such as leptin, adipsin, various other complement components and tumor necrosis factor, suggesting a possible link to the immune system.

Small volumes of Adiponectin / Acrp 30 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS355286 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adiponectin / Acrp 30, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Adiponectin / Acrp 30. The ELISA analytical biochemical technique of the MBS355286 kit is based on Adiponectin / Acrp 30 antibody-Adiponectin / Acrp 30 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Adiponectin / Acrp 30 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Adiponectin / Acrp 30. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).