ALD2 Antibody (HRP)

Cat# orb344872-25ul

Size : 25ul

Brand : Biorbyt

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Phone : +1 850 650 7790

ALD2 Antibody (HRP)

SKU: orb344872

Description

Aldehyde Dehydrogenase antibody (Peroxidase)

Images & Validation

Tested ApplicationsELISA, IP, WB
Dilution RangeELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
ReactivityYeast
Application Notes
Anti-Aldehyde Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAldehyde Dehydrogenase [Yeast]
TargetALD2
PurityAldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.
ConjugationHRP

Storage & Handling

StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

rabbit anti-Aldehyde Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD(P)+] 1, ALD2, ALD5, ALD
  • ALD2 Antibody (HRP) [orb344871]

    ELISA,  IP,  WB

    Yeast

    Rabbit

    Polyclonal

    HRP

    100 μg

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol