Orb699913-500ml | Alternative Block ELISA Blocking Buffer, Synthetic

Alternative Block ELISA Blocking Buffer, Synthetic

SKU: orb699913

Description

ICT’s Alternative Block ELISA Blocking Buffer provides superior background-blocking performance to eliminate interference and minimize background noise on ELISA formats without the use of conventional cross-reactive protein additives.

Images & Validation

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Application Notes	1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Application Notes

1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Key Properties

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Storage & Handling

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Storage	2-8°C
Concentration	1 X
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Alternative Block ELISA Blocking Buffer, Synthetic [orb623179]
Sizes Available: 1 l
Alternative Block ELISA Blocking Buffer, Synthetic [orb1821200]
Sizes Available: 100 ml

Alternative Block ELISA Blocking Buffer, Synthetic

Cat# orb699913-500ml

Contact local distributor :

Brand : Biorbyt

Contact local distributor :

Alternative Block ELISA Blocking Buffer, Synthetic

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Block ELISA Blocking Buffer, Synthetic [orb623179]

Alternative Block ELISA Blocking Buffer, Synthetic [orb1821200]

Protocol Information

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