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> Angiotensin Converting Enzyme (ACE, CD143) (HRP)

Angiotensin Converting Enzyme (ACE, CD143) (HRP)

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Cat# A2295-01D-HRP-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

A2295-01D-HRPAngiotensin Converting Enzyme (ACE, CD143) (HRP)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtP22966
IsotypeIgG1
GradePurified
ApplicationsE IC IHC IP WB
CrossreactivityFe Hm Hu Mk Rt
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.
Immunocytochemistry: Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q2314 (9B9)|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified immunoglobulin|Immunogen: Angiotensin-converting enzyme from human lung.|Specificity: Recognizes N-terminal epitope of human Angiotensin-converting enzyme (ACE). Species Crossreactivity: monkey, rat, hamster and feline. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Angiotensin-converting enzyme from human lung.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified immunoglobulin
Specificity
Recognizes N-terminal epitope of human Angiotensin-converting enzyme (ACE). Species Crossreactivity: monkey, rat, hamster and feline.
References
1. Histochemistry (1987) 87:487–490. 2. Hypertension (1995) 25:219–226. 3. J. Clin. Invest. (1995) 96:1823–1833. 4. J. Nucl. Med. (1989) 30:1686–1692. 5. J. Nucl. Med. (1988) 29:1951–1958. 6. Nucl. Med. Biol. (1993) 20:435–441. 7. Lab. Invest. (1991) 64:118–124. 8. J. Nucl. Med. (1994) 35:1358–1365. 9. Int. Immunol. (1994) 6:1153–1160. 10. PNAS.USA (1996) 93:5213–5218. 11. J. Nucl. Med. (1991) 32:453–460. 12. Lung (1992) 170:349–358. 13. J. Immunol. Methods. (1990) 132:263–273. 14. J. Hypertension (1996) 14: 719–727. 15. J. Biol. Chem. (1994) 269:26806–26814. 16. Hypertension (1999) 33:732–739. 17. Petrov, V., et al., J Renin-Angiotensin-Aldosterone System (2000) 1:342–352.