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> Anti-CD73 Antibody (3D377)

Anti-CD73 Antibody (3D377)

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Cat# NB-64-122876-100uL

Size : 100uL

Brand : Neo Biotech

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Anti-CD73 Antibody (3D377)

(Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)) Copy Product Info

Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)

Catalog No. TMAY-01316 Copy Product Info
Anti-CD73 Antibody (3D377) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (3D377) can be used in ELISA,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-CD73 Antibody (3D377) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (3D377) can be used in ELISA,WB.
SynonymsNTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Ig Type
Mouse IgG2b
Clone
3D377
Reactivity
Human
Specificity
Human 5'-nucleotidase/CD73/NT5E
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Verified Activity
Anti-NT5E mouse monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:63 kDa.
-Observed band size:63 kDa
Application
ELISA
WB
Recommended Dose
WB: 1:500-1:1000; ELISA: 1:5000-1:10000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; TMPY-01851; NP_002517.1; Met 1-Lys 547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research Background5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CD73 / NT5E Protein (TMPY-01851)
Antigen Species
Human
Biology Area
Phosphatases and Regulators
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Keywords

NT-5NT 5CD-73CD 735'-nucleotidase, ecto
Related Tags: Anti-CD73 Antibody (3D377) chemical structure