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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-EpCAM (Extracellular region) Antibody

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Cat# EM0421

Size : 100uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

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Contact local distributor :

Phone : +1 850 650 7790

Anti-EpCAM (Extracellular region) Antibody (M042)

Our EpCAM (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human EpCAM (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Western blot of native lysates including human EpCAM extracellular region (lane 1 & 6), MCF7 breast carcinoma (lane 2 & 7), A431 skin adenocarcinoma (lane 3 & 8), NCI-H1915 lung carcinoma (lane 4 & 9), and NCI-H446 lung carcinoma (lane 5 & 10). The blot was probed with mouse monoclonal anti-EpCAM (EM0391) (lanes 1-5) and mouse monoclonal anti-EpCAM (EM0421) (lanes 6-10) at 1:1000 each.

Immunocytochemical labeling of EpCAM in aldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-EpCAM (EM0421). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-EpCAM (EM0421) for ELISA capture of human recombinant EpCAM extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Western blot of native lysates including human EpCAM extracellular region (lane 1 & 6), MCF7 breast carcinoma (lane 2 & 7), A431 skin adenocarcinoma (lane 3 & 8), NCI-H1915 lung carcinoma (lane 4 & 9), and NCI-H446 lung carcinoma (lane 5 & 10). The blot was probed with mouse monoclonal anti-EpCAM (EM0391) (lanes 1-5) and mouse monoclonal anti-EpCAM (EM0421) (lanes 6-10) at 1:1000 each.

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Species Reactivity

Human

Applications

ICC, IP, WB

Host Species

Mouse

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SKU: EM0421

Ships: 1-2 business days

Product Details

Target

EpCAM (Extracellular region)

Scientific Background

Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that has an extracellular domain with one thyroglobulin type-1 domain and a short cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium. Defects in EpCAM cause hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cell proliferation and differentiation; it up-regulates the expression of FABP5, MYC, and Cyclin A & Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells and in many types of epithelial carcinomas.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M042

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

EPCAM

Molecular Weight

35-45

Immunogen

Clone (M042) was generated from the extracellular region of human EpCAM.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:50
IP: 1:50

Conjugate

Unconjugated

Specificity

Clone M042 mouse monoclonal antibody detects a 35-45 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, H1915, and MCF7 carcinomas. This antibody does not detect denatured EpCAM. The antibody works for native western blot, immunoprecipitation, ELISA, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, Epitelial glycoprotein, EGP, KS 1/4 antigen, KSA, EGP314, CD326, EpCAM

UniProt ID

P16422

Shipping

Blue Ice

Anti-EpCAM (Extracellular region) Antibody

Cat# EM0421

Contact local distributor :

Brand : Phosphosolutions

Contact local distributor :

Anti-EpCAM (Extracellular region) Antibody (M042)

SKU: EM0421

Product Details

Bulk Order Anti-EpCAM (Extracellular region) Antibody