Mouse anti-Human/Cynomolgus Monkey/Rhesus Monkey CD34 Antibody
Anti-Monkey/Rhesus Monkey CD34 Antibody
Anti-Monkey/Rhesus Monkey CD34 Antibody
Contact Us for Bulk Request
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Species | Human |
| Specificity | Does not react with rat. Other species not tested. |
| Host Species | Mouse |
| Accession Number | P28906 |
| Gene Id | 947 |
| Gene Symbols | CD34 |
| Protein Name / Synonyms | Hematopoietic progenitor cell antigen CD34 (CD antigen CD34) |
| Immunogen | A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G purified antibody |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | FACS: use antibody at 0.5-1ug/million cells IF: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cell surface |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85% of angiosarcoma and Kaposis sarcoma, but with a lower specificity.
Storage
Store the CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.