Anti-Mouse CD90.2 (Thy 1.2) [Clone 30-H12] - PE
Cat# C286-200
Size : 200ug
Brand : Leinco Technologies
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AntiMouse CD90.2 (Thy 1.2) [Clone 30H12] — PE
AntiMouse CD90.2 (Thy 1.2) [Clone 30H12] — PE
Product No.: C286
Clone 30H12 Target CD90.2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Thy1.2 Isotype IgG2b Applications FA , FC |
Data
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Product Concentration 0.2 mg/ml Formulation This Rphycoerythrin (RPE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This RPE conjugate is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day 28°C Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/YellowGreen Laser (561 nm) RRIDAB_2829615 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 30H12 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? IHC (Frozen) Costim Depletion For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 30H12 recognizes the Thy1.2 leukocyte marker. Background CD90 is a 2830 kD GPIlinked membrane glycoprotein and is part of the Ig superfamily. It interacts with CD45 in signal transduction. CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that CD90 binds with β2 and β3 integrins and is involved in the inhibition of hematopoietic stem cells proliferation and differentiation, as well as the regulation of cell adhesion and neurite outgrowth. It can be used as a marker for various stem cells, such as hematopoietic stem cells, and for the axonal processes of mature neurons. For use in FACS, CD90 is a popular surface marker for stem cells in combination with other markers such as CD34. There are two alleles for CD90 in mice that differ by one amino acid. The difference being that CD90.1 (Thy1.1) has an arginine and CD90.2 (Thy1.2) has a glutamine at position 108. CD90.2 is more prevalent and is expressed in most mice strains. CD90.1 is only expressed by a select few mice strains including AKR/J and PL strains. CD90.2 is a 2535 kD GPIanchored membrane glycoprotein. Like CD90, it is also in the Ig superfamily, interacts with CD45, and has involvement in signal transduction. The function of CD90.2 is thought to play roles in cognition, axon growth, T lymphocyte function, and apoptosis. CD90 acts as tumor suppressor for some tumors due to its action in upregulating thrombospondin, SPARC (osteonectin), and fibronectin. On the other hand, it has been suspected to aid in the spread of circulating melanoma cells. Regarding prostate cancer, CD90 has therapeutic potential for specific drug targeting due to its expression in cancer associated stroma, but not in normal stroma. Antigen Distribution CD90.2 is present on hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy1.2 bearing mice. Ligand/Receptor CD45 PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Ledbetter, J. and Herzenberg, L. (1979) Immunol. Rev. 47:63 2. Unkeless, J. C. (1979) J. Exp. Med. 150:580 |