Anti-Neurexin 1 antibody
Cat# MBS1750655-0,1mg
Size : 0.1mg
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Figure 1. Western blot analysis of Neurexin 1 using anti-Neurexin 1 antibody (MBS1750655).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SHG-4 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurexin 1 at approximately 170KD. The expected band size for Neurexin 1 is at 162KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SHG-4 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurexin 1 at approximately 170KD. The expected band size for Neurexin 1 is at 162KD.
Figure 2. Flow Cytometry analysis of U251 cells using anti-NRXN1 antibody (MBS1750655).
Overlay histogram showing U251 cells stained with MBS1750655 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN1 Antibody (MBS1750655,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing U251 cells stained with MBS1750655 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN1 Antibody (MBS1750655,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Neurexin 1 (NRXN1) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750655Description: Neurexin-1-alpha is a protein that in humans is encoded by the NRXN1 gene. This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form… More details
Product Overview
Product Name
Neurexin 1 (NRXN1), Polyclonal Antibody
Full Product Name
Anti-Neurexin 1 antibody
Product Gene Name
anti-NRXN1 antibody
Product Synonym Names
Neurexin-1-beta; Neurexin I-beta; NRXN1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4
Purity/Purification
Immunogen affinity purified
Preparation and Storage
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
OMIM
AF064842 mRNA
Immunogen
E Coli-derived human Neurexin 1 recombinant protein (Position: D141-E294). Human Neurexin 1 shares 100% amino acid (aa) sequence identity with both mouse and rat Neurexin 1.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NRXN1 antibody
ELISA (EIA), Flow Cytometry (FC), Western Blot (WB)
Application Notes for anti-NRXN1 antibody
Western Blot (WB):
Concentration: 0.1-0.5µg/ml
Flow Cytometry (FC):
Concentration: 1-3ug / 1X106 cells
Direct ELISA (EIA):
Concentration: 0.1-0.5ug/mL
Tested species: In-house tested species with positive results.
Other applications have not been tested
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5µg/ml
Flow Cytometry (FC):
Concentration: 1-3ug / 1X106 cells
Direct ELISA (EIA):
Concentration: 0.1-0.5ug/mL
Tested species: In-house tested species with positive results.
Other applications have not been tested
Optimal dilutions should be determined by end users.
Western Blot (WB) of anti-NRXN1 antibody
Figure 1. Western blot analysis of Neurexin 1 using anti-Neurexin 1 antibody (MBS1750655).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SHG-4 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurexin 1 at approximately 170KD. The expected band size for Neurexin 1 is at 162KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SHG-4 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurexin 1 at approximately 170KD. The expected band size for Neurexin 1 is at 162KD.
Flow Cytometry (FC/FACS) of anti-NRXN1 antibody
Figure 2. Flow Cytometry analysis of U251 cells using anti-NRXN1 antibody (MBS1750655).
Overlay histogram showing U251 cells stained with MBS1750655 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN1 Antibody (MBS1750655,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing U251 cells stained with MBS1750655 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN1 Antibody (MBS1750655,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Other Notes
Small volumes of anti-NRXN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NRXN1 purchase
MBS1750655 is an antibody targeting NRXN1. Also known as Anti-Neurexin 1 antibody. Reactive in Human. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for NRXN1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
neurexin-1 isoform beta3
NCBI Official Synonym Full Names
neurexin 1
NCBI Official Symbol
NRXN1
NCBI Official Synonym Symbols
PTHSL2; SCZD17; Hs.22998
UniProt Protein Name
Neurexin-1-beta
UniProt Synonym Protein Names
Neurexin I-beta
Protein Family
Neurexin
UniProt Gene Name
NRXN1
NCBI Protein Information
neurexin-1
NCBI Summary for NRXN1
This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2 and may contribute to susceptibility to schizophrenia. [provided by RefSeq, Aug 2016]
Show more UniProt Comments for NRXN1
Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-NRXN1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Extracellular Matrix Organization Pathway antibodies | Extracellular Matrix Organization Pathway Diagram |
| Interactions Of Neurexins And Neuroligins At Synapses Pathway antibodies | Interactions Of Neurexins And Neuroligins At Synapses Pathway Diagram |
| Neuronal System Pathway antibodies | Neuronal System Pathway Diagram |
| Non-integrin Membrane-ECM Interactions Pathway antibodies | Non-integrin Membrane-ECM Interactions Pathway Diagram |
| Protein-protein Interactions At Synapses Pathway antibodies | Protein-protein Interactions At Synapses Pathway Diagram |
Diseases associated with anti-NRXN1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Schizophrenia Antibodies | >90 publications with NRXN1 and Schizophrenia |
| Autism Spectrum Disorder Antibodies | >79 publications with NRXN1 and Autism Spectrum Disorder |
| Autistic Disorder Antibodies | >52 publications with NRXN1 and Autistic Disorder |
| Intellectual Disability Antibodies | >44 publications with NRXN1 and Intellectual Disability |
| Cognition Disorders Antibodies | >15 publications with NRXN1 and Cognition Disorders |
| Abnormalities, Multiple Antibodies | >15 publications with NRXN1 and Abnormalities, Multiple |
| Learning Disorders Antibodies | >12 publications with NRXN1 and Learning Disorders |
| Disease Models, Animal Antibodies | >11 publications with NRXN1 and Disease Models, Animal |
| Language Development Disorders Antibodies | >10 publications with NRXN1 and Language Development Disorders |
| Memory Disorders Antibodies | >10 publications with NRXN1 and Memory Disorders |
Organs/Tissues associated with anti-NRXN1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Nerve Antibodies | >137 publications with NRXN1 and Nerve |
| Brain Antibodies | >99 publications with NRXN1 and Brain |
| Embryonic Tissue Antibodies | >9 publications with NRXN1 and Embryonic Tissue |
| Muscle Antibodies | >6 publications with NRXN1 and Muscle |
| Eye Antibodies | >3 publications with NRXN1 and Eye |
| Lung Antibodies | >2 publications with NRXN1 and Lung |
| Liver Antibodies | >1 publications with NRXN1 and Liver |
| Intestine Antibodies | >1 publications with NRXN1 and Intestine |
| Kidney Antibodies | >1 publications with NRXN1 and Kidney |
| Skin Antibodies | >1 publications with NRXN1 and Skin |
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