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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Neurofilament NF-L Antibody

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Cat# 1453-NFLt

Size : 25uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

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Contact local distributor :

Phone : +1 850 650 7790

Anti-Neurofilament NF-L Antibody

Our Anti-Neurofilament NF-L primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-L and is total IgY fraction. It is great for use in WB, IHC, ICC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (Cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (Cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.

Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody (Cat. 1453-NFL, red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Click on image to zoom

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 1453-NFL

Ships: 1-2 business days

Product Details

Target

Neurofilament NF-L

Scientific Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFL

Molecular Weight

68 kDa

Immunogen

Preparation of bovine spinal cord NF-L.

Immunogen Species

Bovine

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2492162

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN₃

Dilution Ranges

WB: 1:10000
WB Brain: 1:10000
IHC: 1:2000
ICC: 1:2000-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~68 kDa Neurofilament L. Works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

68 kDa neurofilament protein antibody, 68kDa Neurofilament antibody, 68kDa neurofilament protein antibody, CMT1F antibody, CMT2E antibody, FLJ53642 antibody, Light molecular weight neurofilament protein antibody, NEFL antibody, Neurofilament light antibody, Neurofilament light polypeptide 68kDa antibody, Neurofilament light polypeptide antibody, Neurofilament protein, light chain antibody, Neurofilament subunit NF L antibody, Neurofilament triplet L protein antibody, NF-L antibody, NF68 antibody, NFL antibody, NFL_HUMAN antibody

UniProt ID

P02548

NCBI Gene ID

4747

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Research 30:47-62. PMID: 10841809
Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. American Journal of Human Genetics 67:37-46. PMID: 1724473

Anti-Neurofilament NF-L Antibody

Cat# 1453-NFLt

Contact local distributor :

Brand : Phosphosolutions

Contact local distributor :

Anti-Neurofilament NF-L Antibody

SKU: 1453-NFL

Product Details

General References

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