General Info
| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-NLR family CARD domain-containing protein 4 (481-531 aa) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | NLRC4 |
| Gene ID: | 58484 |
| Uniprot ID: | NLRC4_HUMAN |
| Immunogen Region: | 481-531 aa |
| Specificity: | This antibody detects endogenous levels of NLRC4 at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human NLRC4 at the amino acid range 481-531 |
Description
| Post Translational Modifications | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro. |
| Function | Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. |
| Protein Name | Nlr Family Card Domain-Containing Protein 4Card - Lrr - And Nacht-Containing ProteinCed-4-Like Protein ClanCaspase Recruitment Domain-Containing Protein 12Ice Protease-Activating FactorIpaf |
| Database Links | Reactome: R-HSA-6803207Reactome: R-HSA-844623 |
| Cellular Localisation | CytoplasmCytosolInflammasome |
| Alternative Antibody Names | Anti-Nlr Family Card Domain-Containing Protein 4 antibodyAnti-Card - Lrr - And Nacht-Containing Protein antibodyAnti-Ced-4-Like Protein Clan antibodyAnti-Caspase Recruitment Domain-Containing Protein 12 antibodyAnti-Ice Protease-Activating Factor antibodyAnti-Ipaf antibodyAnti-NLRC4 antibodyAnti-CARD12 antibodyAnti-CLAN antibodyAnti-CLAN1 antibodyAnti-IPAF antibodyAnti-UNQ6189 antibodyAnti-PRO20215 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance