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> Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)

Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)

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Cat# NB-64-123165-100uL

Size : 100uL

Brand : Neo Biotech

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Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)

(Synonyms: PRKM3, Phospho-ERK1/2 (T202, Y204), p-ERK1/2 (Thr202, Tyr204), p-ERK1/2 (T202, Y204), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr202, Tyr204), ERK1/2 (p-T202, Y204), ERK-1, ERK1) Copy Product Info

Synonyms: PRKM3, Phospho-ERK1/2 (T202, Y204), p-ERK1/2 (Thr202, Tyr204), p-ERK1/2 (T202, Y204), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr202, Tyr204), ERK1/2 (p-T202, Y204), ERK-1, ERK1

Catalog No. TMAY-01512 Copy Product Info
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr202, Tyr204). Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr202, Tyr204). Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) can be used in WB.
SynonymsPRKM3, Phospho-ERK1/2 (T202, Y204), p-ERK1/2 (Thr202, Tyr204), p-ERK1/2 (T202, Y204), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr202, Tyr204), ERK1/2 (p-T202, Y204), ERK-1, ERK1
Ig Type
Rabbit IgG
Clone
4P145
Reactivity
Human
Specificity
Human, mouse
Species predicted to react based on 100% sequence homology: RatPhospho-ERK1/2 (Thr202, Tyr204)
Verified Activity
1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody (upper), or Anti-ERK2 rabbit polyclonal antibody at 1:1000 dilution (lower).
2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment)
3. Western blot analysis of 15 μg extracts from serum-starved NIH-3T3, untreated (-); treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-ERK1/2 (Thr202, Tyr204) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment)
4. Western blot analysis of extracts from serum-starved Hela, treated with Calyculin A (100 nM, 30 min), using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody and other brands’ antibodies (company C) at dilution of 1:1000, 1:10000 and 1:200000. (Validation Experiment)
Application
WB
Recommended Dose
WB: 1:1000-1:20000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr202, Tyr204 of Human Phospho-ERK1/2.
PurificationProtein A
AppearanceLiquid
Formulation10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Research BackgroundMAPK3 (Mitogen-Activated Protein Kinase 3, also known as ERK1) is a Protein Coding gene. The protein encoded by the ERK1 gene is a member of the MAP kinase family. 3 alternatively spliced human isoforms have been reported. The protein is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs that play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. ERK1 is widely expressed in the small intestine, colon, and other tissues. Diseases associated with MAPK3 include Neutrophil Migration and Pertussis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: residues around Thr202, Tyr204 of Human Phospho-ERK1/2.
Antigen Species
Human
Biology Area
Cancer Drug Targets
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Keywords

pERK1/2 (Thr202, Tyr204)pERK1/2 (T202, Y204)ERK1/2 (pThr202, Tyr204)ERK1/2 (pT202, Y204)ERK1/2 (phospho-Thr202, Tyr204)ERK1/2 (phospho-T202, Y204)
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