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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Semaphorin-3A (N-terminal) Antibody

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Cat# SP1401

Size : 100uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

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Contact local distributor :

Phone : +1 850 650 7790

Anti-Semaphorin-3A (N-terminal) Antibody

Our Semaphorin-3A (N-terminal) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Semaphorin-3A (N-terminal) and is antigen affinity purified. It is great for use in WB, ICC.

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.

Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (SP1401), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: SP1401

Ships: 1-2 business days

Product Details

Target

Semaphorin-3A (N-terminal)

Scientific Background

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SEMA3A

Molecular Weight

Immunogen

Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human Sema3A. The sequence used is identical to similar regions in rat and mouse Sema3A, and has low homology to other semaphorin family members.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera. This antibody has not been shown to be reactive in neonatal rat brain, but has weak reactivity in adult mouse brain.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Sema3A, Semaphorin III

UniProt ID

Q14563

Shipping

Blue Ice

Anti-Semaphorin-3A (N-terminal) Antibody

Cat# SP1401

Contact local distributor :

Brand : Phosphosolutions

Contact local distributor :

Anti-Semaphorin-3A (N-terminal) Antibody

SKU: SP1401

Product Details

Bulk Order Anti-Semaphorin-3A (N-terminal) Antibody