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> Anti-SNAI1 Antibody (6G916)

Anti-SNAI1 Antibody (6G916)

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Cat# NB-64-162917-50uL

Size : 50uL

Brand : Neo Biotech

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Phone : +1 850 650 7790

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Anti-SNAI1 Antibody (6G916)

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Synonyms:

Catalog No. TMAB-12994 Copy Product Info
Anti-SNAI1 Antibody (6G916) is a Rabbit antibody targeting SNAI1. Anti-SNAI1 Antibody (6G916) can be used in FCM, WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-SNAI1 Antibody (6G916) is a Rabbit antibody targeting SNAI1. Anti-SNAI1 Antibody (6G916) can be used in FCM, WB.
Ig Type
IgG
Clone
6G916
Reactivity
Human
Verified Activity
1. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-SNAI1 antibody (TMAB-12994, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 monoclonal antibody, unconjugated (TMAB-12994) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
FCM
WB
Recommended Dose
FCM=1 μg/Test; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
Tissue SpecificityExpressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
ConstructionRecombinant Antibody
PurificationProtein A purified
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
Recombinant Protein: human SNAI1 protein
Gene Name
SNAI1
Gene ID
6615
Protein Name
Zinc finger protein SNAI1
Uniprot ID
O95863
Function
Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.
Chemical Properties
Molecular WeightTheoretical: 32 kDa. Actual: 28 kDa.
Stability & Storage
TransportShipping with blue ice.

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