Anti-SOD1 Antibody

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Size : 30ul

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Western blot analysis of SOD1 expression in mouse liver (A), mouse testis (B), rat liver (C), rat testis (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 16 kD)
Immunofluorescent analysis of SOD1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-SOD1 Antibody
Cat No:	CPA2092
Source:	Rabbit
Reactivity:	H, M, R, B, Mk, Rb, S
Applications:	WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to SOD1

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of SOD1 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Gene Symbol：SOD1

Alternative Names：Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1

Entrez Gene (Human)： 6647;

Entrez Gene (Mouse)： 20655;

Entrez Gene (Rat)： 24786;

SwissProt (Human)： P00441;

SwissProt (Mouse)： P08228;

SwissProt (Rat)： P07632;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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