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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Synaptobrevin (VAMP) Antibody

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Cat# 1933-SYBt

Size : 25uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

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Contact local distributor :

Phone : +1 850 650 7790

Anti-Synaptobrevin (VAMP) Antibody (SP10)

Our Anti-Synaptobrevin (VAMP) primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, pig, rat, rabbit, and hamster Synaptobrevin (VAMP) and is IgM purified culture supernatant. It is great for use in WB, IHC.

Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein.

Western blot showing specific immunolabeling of Synaptobrevin (VAMP, cat. 1933-SYB, 1:1000) in mouse brain, rat brain, and lamprey spinal cord. Image kindly provided by Karina Vargas, University of Pittsburgh.

Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein.

Click on image to zoom

Species Reactivity

Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat

Applications

IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1933-SYB

Ships: 1-2 business days

Product Details

Target

Synaptobrevin (VAMP)

Scientific Background

Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive deficits in Alzheimer’s disease (Sze et al., 2000).

Format

IgM Purified culture supernatant

Clonality

Monoclonal

Clone

SP10

Isotype

IgM

Applications

IHC, WB

Host Species

Mouse

Gene Name

VAMP1/2

Molecular Weight

16 kDa

Immunogen

Synaptic immunoprecipitate (crude) from human brain.

Immunogen Species

Human

Species Reactivity

Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat

Antibody Registry ID

AB_2492248

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

IgM purified culture supernatant.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~16 kDa synaptobrevin. The antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SYB antibody, SYB1 antibody, Synaptobrevin 1 antibody, VAMP antibody, VAMP1 antibody, Vesicle associated membrane protein 1 antibody

UniProt ID

P23763

NCBI Gene ID

6843

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Deák F, Schoch S, Liu X, Südhof TC, Kavalali ET (2004) Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nature Cell Biology 2004 Nov;6(11):1102-8. PMID: 15475946
Sze CI, Bi H, Kleinschmidt-DeMasters BK, Filley CM, Martin LJ (2000) Selective regional loss of exocytotic presynaptic vesicle proteins in Alzheimer's disease brains. Journal of the Neurological Sciences 175(2):81-90. PMID: 10831767
Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex (1993). Brain Research 609, 9-20. PMID: 7685234

Anti-Synaptobrevin (VAMP) Antibody

Cat# 1933-SYBt

Contact local distributor :

Brand : Phosphosolutions

Contact local distributor :

Anti-Synaptobrevin (VAMP) Antibody (SP10)

SKU: 1933-SYB

Product Details

General References

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