Antithrombin III (ATIII) (FITC)

Antithrombin III (ATIII) is a plasma protein synthesized in the liver. Normal plasma levels are 115-160mg/L. ATIII consists of a single polypeptide chain and has a molecular weight of 58kD. ATIII is a serine protease inhibitor and through this action regulates blood coagulation. ATIII also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000-fold.

Applications:
Suitable for use in Radial Immunodiffusion (RID), Double Diffusion and Immunoelectrophoresis. Other applications have not been tested.

Recommended Dilutions:
RID: 6ul antiserum/cm2 gel vs. 5ul neat - 1:10 dilution human plasma.
Double Diffusion: 10ul antiserum vs. 5ul plasma
IEP: 100ul antiserum vs. 5ul plasma gives single arc.
Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.