beta-Actin Antibody
Cat# MBS9602458-0,05mL
Size : 0.05mL
Brand : MyBiosource
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Phone : +1 850 650 7790
Figure 1: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Beta-Actin (ACTB) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9602458Description: Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly… More details
Product Overview
Online Datasheet in PDFProduct Name
beta-Actin (ACTB), Monoclonal Antibody
Full Product Name
beta-Actin Antibody
Product Gene Name
anti-ACTB antibody
Product Synonym Names
A26C1A; A26C1B; ACTB; ACTB_HUMAN; Actin beta; Actin cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actx; b actin; Beta cytoskeletal actin; Beta-actin; BRWS1; E430023M04Rik; MGC128179; PS1TP5 binding protein 1; PS1TP5BP1
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Monkey, Hamster
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Concentration
1mg/ml (lot specific)
Specificity
beta-Actin antibody detects endogenous levels of total beta-Actin
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
beta-Actin peptide (MBS9619911) is used for blocking the activity of beta-Actin antibody (MBS9602458)
Molecular Weight
Observed: 43 kD
Predicted: 42 kDa
Predicted: 42 kDa
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
Purified recombinant fragment of human beta-Actin expressed in E. Coli
Subcellular Location
Cytoplasm > Cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ACTB antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-ACTB antibody
ELISA: 1:10000
WB: 1:500-1:2000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
Product References and Citations for anti-ACTB antibody
Wu D, Huang CJ, Jiao XF, Ding ZM, Zhang JY, Chen F, Wang YS, Li X, Huo LJ; Journal: Oncotarget. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Xie Z, Qu Y, Leng Y, Sun W, Ma S, Wei J, Hu J, Zhang X; Journal: Drug Des Devel Ther. Human colon carcinogenesis is associated with increased interleukin-17-driven inflammatory responses. Tong Lu, Huayou Luo, Jia Liu, Cheng Chen, Feng Wang, Zongwen Shen, Tonglei Liu, Yujian Zeng, Ting-Hua Wang, Kun-Hua Wang.
Other Notes
Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ACTB purchase
MBS9602458 is an antibody targeting ACTB. Also known as beta-Actin Antibody. Reactive in Human, Mouse, Rat, Monkey, Hamster. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
Actin
UniProt Gene Name
ACTB
NCBI Protein Information
actin, cytoplasmic 1
NCBI Summary for ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
Show more UniProt Comments for ACTB
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-ACTB antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Adherens Junction Pathway antibodies | Adherens Junction Pathway Diagram |
| Adherens Junction Pathway antibodies | Adherens Junction Pathway Diagram |
| Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies | Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram |
| Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies | Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram |
| Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies | Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram |
| Bacterial Invasion Of Epithelial Cells Pathway antibodies | Bacterial Invasion Of Epithelial Cells Pathway Diagram |
| Bacterial Invasion Of Epithelial Cells Pathway antibodies | Bacterial Invasion Of Epithelial Cells Pathway Diagram |
| Chaperonin-mediated Protein Folding Pathway antibodies | Chaperonin-mediated Protein Folding Pathway Diagram |
| Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies | Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram |
| Dilated Cardiomyopathy Pathway antibodies | Dilated Cardiomyopathy Pathway Diagram |
Diseases associated with anti-ACTB antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >83 publications with ACTB and Neoplasms |
| Carcinoma Antibodies | >39 publications with ACTB and Carcinoma |
| Liver Diseases Antibodies | >17 publications with ACTB and Liver Diseases |
| Disease Models, Animal Antibodies | >16 publications with ACTB and Disease Models, Animal |
| Brain Diseases Antibodies | >13 publications with ACTB and Brain Diseases |
| Lung Diseases Antibodies | >12 publications with ACTB and Lung Diseases |
| Necrosis Antibodies | >12 publications with ACTB and Necrosis |
| Liver Neoplasms Antibodies | >11 publications with ACTB and Liver Neoplasms |
| Kidney Diseases Antibodies | >11 publications with ACTB and Kidney Diseases |
| Carcinoma, Hepatocellular Antibodies | >9 publications with ACTB and Carcinoma, Hepatocellular |
Organs/Tissues associated with anti-ACTB antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >54 publications with ACTB and Blood |
| Brain Antibodies | >46 publications with ACTB and Brain |
| Liver Antibodies | >43 publications with ACTB and Liver |
| Muscle Antibodies | >40 publications with ACTB and Muscle |
| Kidney Antibodies | >24 publications with ACTB and Kidney |
| Lung Antibodies | >20 publications with ACTB and Lung |
| Bone Antibodies | >18 publications with ACTB and Bone |
| Heart Antibodies | >18 publications with ACTB and Heart |
| Embryonic Tissue Antibodies | >14 publications with ACTB and Embryonic Tissue |
| Skin Antibodies | >13 publications with ACTB and Skin |