Brachyury (TBXT, T-box Transcription Factor T, Protein T)

The Brachyury protein is required for the proper formation and differentiation of posterior mesoderm and for axial development in all vertebrates. The Brachyury gene encodes a transcription factor that binds to a specific DNA element via its N-terminal region. A protein motif within the DNA-binding domain, the so-called T box, is highly conserved among T homologs from different species and also defines a broader family of T-box genes Brachyury functions during the earlier stages of gastrulation, which suggests that at least one of its early roles is to regulate the morphogenetic behavior of nascent mesoderm by altering cell autonomous properties.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.