CAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (FITC)

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Cat# 217244-FITC-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

217244-FITCCAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (FITC)

Specifications

Clone TypePolyclonal

Hostrabbit

Sourcerat

Swiss ProtP11275

IsotypeIgG

GradeAffinity Purified

ApplicationsWB

CrossreactivityBo Ch Gp Hm Hu Mo Rb Rt

Shipping TempBlue Ice

Storage Temp-20°C

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Control:
HEK293 cell lysate

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Pab|Isotype: IgG|Host: rabbit|Source: rat|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to the N-terminal of rat CaMK II.|Specificity: Recognizes rat CaMK II. Species Crossreactivity: human, mouse, bovine, hamster, guinea pig, chicken and rabbit.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Synthetic peptide corresponding to the N-terminal of rat CaMK II.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes rat CaMK II. Species Crossreactivity: human, mouse, bovine, hamster, guinea pig, chicken and rabbit.

References

1. Downregulation of delta CaM kinase II in human tumor cells.Tombes, R M, et al. (1999) Biochim. Biophys. Acta, 1452: 1-11 (1999). 2. Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation. Koh, Y H, et al. (1999) Cell, 98: 353-63 (1999). 3. Inhibition of calcium/calmodulin-dependent protein kinase II in rat hippocampus attenuates morphine tolerance and dependence. Fan, G H, et al. (1999) Mol. Pharmacol., 56: 39-45 (1999). 4. Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium. Hoch, B, et al. (1999) Circ. Res., 84: 713-21 (1999). 5. Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. Erondu, N E and Kennedy, M B (1985) J. Neurosci., 5: 3270-7 (1985). 6. AlphaCaMKII binding to the C-terminal tail of NMDA receptor subunit NR2A and its modulation by autophosphorylation. Gardoni, F, et al. (1999) FEBS Lett., 456: 394-8 (1999). 7. CaMKII regulates the density of central glutamatergic synapses in vivo. Rongo, C and Kaplan, J M (1999) Nature, 402: 195-9 (1999).