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> Catenin, beta (Biotin)

Catenin, beta (Biotin)

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Cat# C2069-50D-Biotin-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2069-50D-BiotinCatenin, beta (Biotin)

Specifications
Clone TypePolyclonal
Hostrabbit
Sourcehuman
Swiss ProtP35222
IsotypeIgG
GradeAffinity Purified
ApplicationsE IP WB
CrossreactivityCh Hu Mo Rt Xe
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1.0ug/ml
Western Blot after IP: 1-2ug/ml. Crossreactivity with a ~100kD protein of unknown identity is sometimes observed.
Immunoprecipitation: 5-10ug. Specifically immunoprecipitate the ~ 92kD b-Catenin protein from native cell lysates.
Optimal dilutions to be determined by the researcher.

Positive Controls:
HeLa and A431 lysates.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography.|Immunogen: A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein. |Specificity: Recognizes human b-catenin. Species Crossreactivity: chicken, mouse, rat and Xenopus..||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human b-catenin. Species Crossreactivity: chicken, mouse, rat and Xenopus..
References
1. Johnson, K.R., et al; Exp. Cell Res. 207:252–260 (1993). 2. Geiger, B., et al; Curr. Opin. Cell Biol. 1:103–109 (1989). 3. Peifer, M., et al; Science 262:1667–1668 (1993). 4. Takeichi, M., et al; Science 51:1451–1455 (1991). 5. Daniel, J.M., et al; Mol. Cell. Biol. 270:4819–4824 (1995). 6. Hinck, L, et al; J. Cell. Biol. 125:1327–1340 (1994). 7. Knudsen, K.A., et al; J. Cell. Biol. 18:671–679 (1992). 8. McCrea, P.D., et al; J. Biol. Chem. 266:4514–4520 (1991). 9. Ozawa, M., et al; Proc. Natl Acad. Sci. USA. 87:4246–4250 (1990). 10. Knudsen, K.A., et al; J. Biol. Chem. 130:67–77 (1995). 11. Ozawa, M., et al; J. Cell. Biol. 116:989–996 (1992). 12. McCrea, P.D., et al; Science 25:1359–1361 (1991). 13. Peifer, M., et al; Cell 63:1167–1178 (1990). 14. Peifer, M., et al; Cell 76:789–791 (1994). 15. Rubinfeld, B., et al; Science 262:1731–1734 (1993). 16. Su, L.-K., et al; Science 262:1734–1737 (1993). 17. Kowalczk, A.P., et al; J. Biol. Chem. 269:31214–31223 (1994)