CD14 (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein) (PerCP/Cy5.5)

Cat# 367028-200T

Size : 200Tests

Brand : US Biological

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Phone : +1 850 650 7790


367028CD14 (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein) (PerCP/Cy5.5)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtP08572
IsotypeIgG2b,k
GradeHighly Purified
Accession #NM_000591.4
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

CD14 is a 53-55kD glycosylphosphatidylinositol (GPI)-anchored and single chain glycoprotein expressed at high levels on monocytes. Additionally, CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells. The binding of CD14 antibody does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.

Applications
Product Type: Mab|Isotype: IgG2b,k|Clone No: 26ic|Host: mouse|Source: human|Concentration: ~0.025mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with PerCP/Cy5.5. |Purity: Highly Purified|Immunogen: Cultured human peripheral blood monocytes|Specificity: Recognizes human CD14. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Cultured human peripheral blood monocytes
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with PerCP/Cy5.5.
Purity
Highly Purified
Specificity
Recognizes human CD14.
References
1. Antal-Szalmas P, et al. 1997. Leukoc Biol. 61:721. 2. Antal-Szalmas P, et al. 2001. Cytometry. 45:115. 3. Bate C, et al. 2005. J Neuroimmunol. 170:62. 4. Detmers PA, et al. 1998. J Immunol. 161:1921. 5. Giambartolomei GH, et al. 1999. Infect Immun. 67:140.