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> CD146/MCAM Protein, Human, Recombinant (hFc)

CD146/MCAM Protein, Human, Recombinant (hFc)

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Cat# NB-64-56069-20ug

Size : 20ug

Brand : Neo Biotech

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CD146/MCAM Protein, Human, Recombinant (hFc)

(Synonyms: MUC18, melanoma cell adhesion molecule, CD146) Copy Product Info

Synonyms: MUC18, melanoma cell adhesion molecule, CD146

Catalog No. TMPY-00879 Copy Product Info
CD146/MCAM Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 86.4 kDa and the accession number is A0A024R3I5.
Other Forms of CD146/MCAM Protein, Human, Recombinant (hFc):
CD146/MCAM Protein, Human, Recombinant (His)
CD146/MCAM Protein, Mouse, Recombinant (His)
CD146/MCAM Protein, Human, Recombinant (His), Biotinylated
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD146/MCAM Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 86.4 kDa and the accession number is A0A024R3I5.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP43121
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human CD146 precursor (NP_006491.2) was expressed with the C-terminal fused Fc region of human IgG1. Predicted N terminal: Val 24
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsMUC18, melanoma cell adhesion molecule, CD146
Research Background
The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas.
Chemical Properties
Molecular Weight86.4 kDa (predicted); 120-130 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

MUC-18MUC 18CD-146CD 146
Related Tags: CD146/MCAM Protein, Human, Recombinant (hFc) chemical structure | CD146/MCAM Protein, Human, Recombinant (hFc) molecular weight