Reagents and instruments for immunology, cell biology and molecular biology.
 
> CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4) (PE)

CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4) (PE)

Print Print
Please log in

Cat# C2255-02B3-100T

Size : 100Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2255-02B3CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4) (PE)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcesheep
IsotypeIgG2a
GradeAffinity Purified
ApplicationsFC
CrossreactivityGt Sh
Shipping TempBlue Ice
Storage Temp4°C Do not freeze

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line.

Recommended Negative Control:
I1904-84B2: IgG2a Negative Control, Murine (PE)

Storage and Stability:
Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 10B1533 (44.38)|Host: mouse|Source: sheep|Concentration: Not Determined|Form: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by Protein G affinity chromatography.|Immunogen: Fetal thymocytes|Specificity: Recognizes sheep CD4. Species Crossreactivity: goat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Fetal thymocytes
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes sheep CD4. Species Crossreactivity: goat
References
1. Maddox, J. et al. (1985) Immunology. 55: 739-748. 2. Mackay, C. et al. (1986) Eur. J. Immunol. 16: 19-25. 3. Mackay, C. et al. (1987) Cell. Immunol. 110: 46-55. 4. Mackay, C. et al. (1986) J. Immunol. 136 (5): 1592-1599. 5. Breugelmans, S. et al. (2010) Acta Histochem. May 21. 6. Connelley, T. et al. (2011) . Vet Res. 42: 37. 7. Brown, M.N. et al. (2010) J Immunol. 185: 4873-82. 8. Chan, S.S. et al. (2002) Immunology. 107: 366-72. 9. Foulon, E. et al. (2008) J Immunol Methods. 339: 1-10. 10. Debes, G.F. et al. (2005) . Nat Immunol. 6: 889-94. 11. Gillan, S. et al. (2010) I Clin Vaccine Immunol. 17: 108-17. 12. Lybeck, K. R. et al. (2009) Clin. Vaccine. Immunol. 16: 1003-11.