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> CD45 (LCA) (PE)

CD45 (LCA) (PE)

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Cat# 214695-PE-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790

214695-PE CD45 (LCA) (PE)

Clone Type
Monoclonal
Host
mouse
Source
chicken
Isotype
IgG2a
Grade
Affinity Purified
Applications
IHC IP
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. Studies have indicated that dephosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn.

Investigations into the properties of CD45 in chicken models are limited, but there is evidence of the existence of an additional cysteine residue near the transmembrane region. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution::
Flow Cytometry: 1:100-1:200, 10ul labels 10e6 cells in 100ul
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control Tissue:
Chicken lung

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: UM16-6|Host: mouse|Source: chicken|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: COS cells transfected with CD45 gene.|Specificity: Recognizes chicken CD45. Does not cross react with turkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
COS cells transfected with CD45 gene.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes chicken CD45. Does not cross react with turkey.
References
1. Pavlova, S. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus. J Gen Virol. 91:847-57. Further Reading 2. Symons, A. et al. (1999) Domain organization of the extracellular region of CD45. Protein Engineering 12(10): 885-892. 3. Okumura, M. et al. (1996) Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains. J. Immunol. 157: 1569-1575. 4. Jung, E.J. et al. (1997) Phosphorylation of chicken protein tyrosine phosphatase 1 by casein kinase II in vitro. Experimental and Molecular Medicine 29(4): 229-233.