Reagents and instruments for immunology, cell biology and molecular biology.
 
> CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (HRP)

CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (HRP)

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Cat# C2256-12D-HRP-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2256-12D-HRPCD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (HRP)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcebovine
Swiss ProtP19238
IsotypeIgG1
GradeAffinity Purified
ApplicationsIHC IP
CrossreactivityBo Gt Sh
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CC17|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant. |Immunogen: Bovine thymocytes.|Specificity: Recognizes bovine CD5. Species Crossreactivity: goat and sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Bovine thymocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD5. Species Crossreactivity: goat and sheep
References
1. Howard, C.J. et al. (1991) Individual antigens of cattle, bovine CD5 (BoCD5). Vet. Immunol. Immunopathol. 27: 55-60. 2. Howard, C.J. et al. (1988) Two monoclonal antibodies (CC17 - CC29) recognizing an antigen (Bo5) on bovine T-lymphocytes, analagous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139. 3. Howard, C.J. et al. (1991) Competitive binding with putative Bo5 (CD5) cluster of monoclonal antibodies. Vet. Immunol. Immunopathol. 27: 147-151.4. Hein, W. R. et al. (1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species. Vet. Immunol. Immunopathol. 27: 32-34.