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> CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58) (PE)

CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58) (PE)

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Cat# C2409-14B-PE-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790

C2409-14B-PECD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58) (PE)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtP05362
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC IHC IP
CrossreactivityHu Po
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

CD54 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein of ~90kD with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections
Optimal working dilutions to be determined by researcher.

Recommended Positive Control:
Human Tonsil

Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H1547 (15.2)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Human monocytes.|Specificity: Recognizes human CD54, a 90kD glycoprotein. Blocks CD54 function. Species Crossreactivity: porcine||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human monocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD54, a 90kD glycoprotein. Blocks CD54 function. Species Crossreactivity: porcine
References
1. J. Cell Biol. (1992) 116: 1527-1535. 2. Cell (1992) 68: 71-81. 3. Treadwell, M.D. et al. (1996). Increased neutrophil adherence to endothelial cells exposed to asbestos. Toxicol Appl. Pharmacol. 139: 62-70.