CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (PE)

Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation (1). Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation (2, 3). Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response (4).

Applications:
Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50 labels 5x10e5 cells
Optimal dilutions to be determined by the researcher.

Flow Cytometry Protocol:
1. Five x 10e5 cultured human Raji cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2433-10D at a dilution factor of 1:50 (10ug/ml).
2. Cells were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 2.11 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration.
4. Binding was blocked when cells were preincubated for 10 minutes with 20ul of 0.5mg/ml C2433-10A.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.