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> CD81 (TAPA-1, Target of anti-Proliferative Antibody-1)

CD81 (TAPA-1, Target of anti-Proliferative Antibody-1)

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Cat# C2433-12A-100ug

Size : 100ug

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2433-12A CD81 (TAPA-1, Target of anti-Proliferative Antibody-1)

Clone Type
Monoclonal
Host
hamster
Source
mouse
Swiss Prot
P35762
Isotype
IgG1
Grade
Affinity Purified
Applications
E FC IHC IP WB
Crossreactivity
Mo Rt Rd
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD81, also known as TAPA-1 or Target of the antiproliferative antibody, is a 236 amino acid ~26kD multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. C2433-12A induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). C2433-12A requires the presence of both extracellular loops of TAPA-1 for binding. Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006).

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry (frozen): The recognized epitope is reported to be sensitive to formaldehyde fixation and tissue processing. Use of acetone fixation is recommened.
Western Blot: non-reducing conditions
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.

Recommended Secondary Reagents:
I1903-04T: IgG H&L (HRP) Pab Gt x Hm
I1903-04Q: IgG H&L (FITC) Pab Gt x Hm

Recommended Negative Controls:
Hamster IgG Negative Control

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H1702 (Eat 2)|Host: hamster|Source: mouse|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: 38C13, murine B cell line.|Specificity: Recognizes mouse CD81. Species Crossreactivity: rat ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
38C13, murine B cell line.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes mouse CD81. Species Crossreactivity: rat
References
1. Clark, K. et al. (2001). PGRL is a major CD81 - associated protein on lymphocytes and distingushes a new family of cell surface proteins. J. Immunol. 167: 5115 - 5121. 2. Maecker, HT et al., Hybridoma 19: 15-22. 3. Conde-Vancells, J. et al. (2010) Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples. Proteomics Clin Appl. 4: 416-425. 4. Conde-Vancells, J. et al. (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res. 7: 5157-66. 5. Takeda, Y. et al. (2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 283: 26089-97. 6. Suzuki, M. et al. (2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation. J Immunol. 182: 6485-93. 7. Ha, C.T. et al. (2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1. J Leukoc Biol. 77: 948-57. 8. Pan, Q. et al. (2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi). Gut. 61: 1330-9. 9. Jin, Y. et al. (2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages. PLoS One. 8: e73706. 10. Royo, F. et al. (2013) Transcriptome of extracellular vesicles released by hepatocytes. PLoS One. 8: e68693. 11. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion. Cancer Res. 61: 5248-54.