CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (Biotin)

CD81 (Mr 25kD) is a member of the transmembrane 4 integral membrane protein family CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse.1 CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma/delta+ T cells to more mature T cells with alpha/beta T cell receptors.2 Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pTalpha.2 This antibody can block thymocyte interaction with CD81 in vitro.2

Applications
• Identification and enumeration of CD81+ cells by flow cytometry 2
• Immunohistochemistry (frozen sections) 2
• Immunoprecipitation 2
• In vitro blocking of thymocyte interactions with CD81 2

Recommended Dilution:
Flow Cytometry: ≤ 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.