Cellulase (Onozuka R-10) is a multi-component enzyme system derived from Trichoderma viride and contains cellulase, alpha-amylase, hemicellulase, pectinase and protease activities. It is often used in the isolation of plant protoplasts in combination with other enzymes.

Source:
Trichoderma viride

Synonyms:
Onozuka R-10

CAS No:
9012-54-8

EC Number:
3.2.1.4

Unit Definition of Activity:
One unit of Cellulase will liberate 1.0 micromole of glucose form carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).

Major Enzyme Activity:
Cellulase: 1u/mg

Extraneous Enzyme Activities:
Pectinase: 0.4u/mg
Hemicellulase: 1.0u/mg
alpha-Amylase: 0.6u/mg
Protease: 0.01u/mg

Optimum pH range:
4-5

Appearance:
Light yellow powder

Solubility:
Soluble in water (10mg/ml)

Loss on Drying:
≤10%

Arsenic:
≤3ug

Heavy Metals:
≤40ug

Activity:
≥10,000U/g

Total Viable Count:
≤10,000cfu/g

Bacteria:
≤10,000cfu/g

Fungi:
<100cfu/g

Coliform:
Negative

Stability and Storage:
Lyophilized powder may be stored at 4°C. Stable for 6 months at 4°C.