Chick Fibroblast Basal Medium MCDB 201 (Powder)
Cat# MBS652663-50L
Size : 50L
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Chick Fibroblast Basal Medium MCDB 201 Culture Media
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652663Basal Medium MCDB 201 is specifically formulated for the cultivation of chick fibroblasts in accordance with the formulation by McKeehan and Ham. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed). Also suitable for stem cell and CHO cell… More details
Product Overview
Online Datasheet in PDFProduct Name
Chick Fibroblast Basal Medium MCDB 201, Culture Media
Full Product Name
Chick Fibroblast Basal Medium MCDB 201 (Powder)
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Endotoxin Level
≤1 EU/ml
Grade
Cell Culture Grade
Appearance
White , homogenous, free flowing powder
pH
5.6
Solubility
Pale yellow, clear, complete
Directions per Liter
Dissolve 17.6g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/liter
Inorganic Salts:
Ammonium Metavanadate: 0.00001
Ammonium Molybdate•H2O: 0.000618
Calcium Chloride•2H20: 294.0
Cupric Sulfate•5H20: 0.00025
Ferrous Sulfate•7H20: 1.668
Magnesium Sulfate: 180.57
Manganese Sulfate: 0.000075
Nickel Chloride•6H2O: 0.0000012
Potassium Chloride: 223.65
Sodium Chloride: 7597.0
Sodium Metasilicate•9H2O: 0.1420
Sodium Phosphate Dibasic•H2O: 70.99
Sodium Selenite: 0.00087
Zinc Sulfate•7H2O: 0.02874
Amino Acids:
L-Alanine 8.90
L-Arginine HCl 63.20
L-Asparagine•H2O: 150
L-Aspartic Acid: 13.31
L-Cysteine•HCl•H2O: 35.13
L-Glutamic Acid: 14.71
L-Glutamine: 146.15
Glycine: 7.51
L-Histidine•HCl•H2O: 20.97
L-Isoleucine: 13.12
L-Leucine: 39.35
L-Lysine•HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 5.76
L-Serine: 31.53
L-Threonine: 35.74
L-Tryptophan: 6.13
L-Tyrosine•Na2•2H2O: 11.35
L-Valine: 35.13
Vitamins:
Biotin: 0.00730
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.00512
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid•Ca: 0.477
Pyridoxine•HCl: 0.0617
Riboflavin: 0.113
Thiamine•HCl: 0.337
Other:
Adenine HCl: 0.172
D-Glucose: 1441.0
HEPES: 7149.0
Linoleic Acid: 0.0841
Lipoic Acid: 0.00206
Phenol Red, Sodium: 1.242
PutrescinE•2HCl: 0.00016
Sodium Pyruvate: 55.00
Thymidine: 0.0727
Total: 17.6g/liter
Ammonium Metavanadate: 0.00001
Ammonium Molybdate•H2O: 0.000618
Calcium Chloride•2H20: 294.0
Cupric Sulfate•5H20: 0.00025
Ferrous Sulfate•7H20: 1.668
Magnesium Sulfate: 180.57
Manganese Sulfate: 0.000075
Nickel Chloride•6H2O: 0.0000012
Potassium Chloride: 223.65
Sodium Chloride: 7597.0
Sodium Metasilicate•9H2O: 0.1420
Sodium Phosphate Dibasic•H2O: 70.99
Sodium Selenite: 0.00087
Zinc Sulfate•7H2O: 0.02874
Amino Acids:
L-Alanine 8.90
L-Arginine HCl 63.20
L-Asparagine•H2O: 150
L-Aspartic Acid: 13.31
L-Cysteine•HCl•H2O: 35.13
L-Glutamic Acid: 14.71
L-Glutamine: 146.15
Glycine: 7.51
L-Histidine•HCl•H2O: 20.97
L-Isoleucine: 13.12
L-Leucine: 39.35
L-Lysine•HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 5.76
L-Serine: 31.53
L-Threonine: 35.74
L-Tryptophan: 6.13
L-Tyrosine•Na2•2H2O: 11.35
L-Valine: 35.13
Vitamins:
Biotin: 0.00730
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.00512
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid•Ca: 0.477
Pyridoxine•HCl: 0.0617
Riboflavin: 0.113
Thiamine•HCl: 0.337
Other:
Adenine HCl: 0.172
D-Glucose: 1441.0
HEPES: 7149.0
Linoleic Acid: 0.0841
Lipoic Acid: 0.00206
Phenol Red, Sodium: 1.242
PutrescinE•2HCl: 0.00016
Sodium Pyruvate: 55.00
Thymidine: 0.0727
Total: 17.6g/liter
Product Categories/Family for Chick Fibroblast Basal Medium MCDB 201 culture media
Culture Media; Media-Cell Culture; MCDB
Other Notes
Small volumes of Chick Fibroblast Basal Medium MCDB 201 culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Chick Fibroblast Basal Medium MCDB 201 purchase
MBS652663 is a culture media targeting Chick Fibroblast Basal Medium MCDB 201. Also known as Chick Fibroblast Basal Medium MCDB 201 (Powder).
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