Size : 500mg
Phone : +1 850 650 7790
Synonyms: NSC-407296, Heriguard, 3-O-Caffeoylquinic acid
| Description | Chlorogenic acid is a natural phenol found in honeysuckle. Chlorogenic acid has a wide range of biological activities including anti-inflammatory, antioxidant, antitumor, antimicrobial, and neuroprotective. |
| Targets & IC50 | SW48 cells:353 μM, HBV DNA:1.2 ± 0.4 μM, HSV1:15.3 μg/mL (EC50), HeLa cells:375 μM, HCT116 cells:367 μM, BALB/3T3 cells:> 280 μM, Caco-2 cells:758 ± 19.09 µM (EC50) |
| In vitro | METHODS: Human colorectal cancer cells Caco-2 were treated with Chlorogenic acid (50-1000 µM) for 24 h and cell viability was measured by MTT assay. RESULTS: Proliferation of Chlorogenic acid-treated cells was significantly reduced, starting at the lowest tested concentration (50 µM), with an EC50 of 758 ± 19.09 µM.[1] METHODS: Mouse mammary carcinoma cells 4T1 were treated with Chlorogenic acid (50-200 µM) for 24 h to enable detection of apoptosis by Flow cytometry. RESULTS: In the early stage, the level of apoptotic cells (Annexin V+/PI-) increased significantly from 1.14% to 0.04%, 34.56%, 40.9% and 37.46% with increasing concentrations of Chlorogenic acid (0, 50, 100, 150 and 200 µM, respectively). Whereas, in the late phase, when the cells were treated with Chlorogenic acid, the apoptotic cell ratio (Annexin V+/PI+) significantly increased from 2.34% to 2.08%, 13.45%, 17.7% and 29.06%, respectively. [2] |
| In vivo | METHODS: To assess the anti-obesity effects, Chlorogenic acid (150 mg/kg) was administered by gavage to ICR mice on a high-fat diet model (HFD) once daily for six weeks. RESULTS: Administration of Chlorogenic acid significantly promoted weight loss, decreased plasma lipid levels, and altered mRNA expression of genes related to adipogenesis and lipolysis in adipose tissue. [2] |
| Synonyms | NSC-407296, Heriguard, 3-O-Caffeoylquinic acid |
| Molecular Weight | 354.31 |
| Formula | C16H18O9 |
| Cas No. | 327-97-9 |
| Smiles | O(C(C=CC1=CC(O)=C(O)C=C1)=O)[C@@H]2C[C@@](C(O)=O)(O)C[C@@H](O)[C@H]2O |
| Relative Density. | 1.3594 g/cm3 (Estimated) |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | Ethanol: 66 mg/mL (186.28 mM), Sonication is recommended. DMSO: 237 mg/mL (668.91 mM), Sonication is recommended.  H2O: 10 mg/mL (28.22 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (14.11 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/Ethanol/DMSO
Ethanol/DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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