Citrate Synthase (CS) Activity Assay Kit (Colorimetric)
Cat# MBS9719200-48
Size : 48Tests
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Citrate Synthase (Cs) Activity (CS) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9719200PROMOTIONAL PRICING: 5% OFF web . Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed
Description: Citrate Synthase (CS) Activity Assay Kit provides a simple, sensitive, rapid and colorimetric CS activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or… More details
Product Overview
Online Datasheet in PDFProduct Name
Citrate Synthase (CS) Activity, Assay Kit
Full Product Name
Citrate Synthase (CS) Activity Assay Kit (Colorimetric)
Product Gene Name
CS assay kit
Preparation and Storage
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Tag
Citrate Synthase, CS
Features & Benefits
simple, sensitive, rapid and colorimetric CS activity detection method.
compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension).
Provides detailed sample preparation and results calculation methods.
Citrate Synthase: CS Activity Assay Kit provides a simple, sensitive, rapid and colorimetric CS activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures: adherent or suspension CS activity is determined depending on that CS can catalyze acetyl CoA and oxamic acid to produce citamoyl co-enzyme A, further hydrolysis to produce citric acid; this reaction causes colorless DTNB to transform into yellow TNB, TNB has a characteristic absorbing value at 412nm.The activity of citrate synthase could be obtained by calculated the increase rate of the light absorption at 412nm.
compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension).
Provides detailed sample preparation and results calculation methods.
Citrate Synthase: CS Activity Assay Kit provides a simple, sensitive, rapid and colorimetric CS activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures: adherent or suspension CS activity is determined depending on that CS can catalyze acetyl CoA and oxamic acid to produce citamoyl co-enzyme A, further hydrolysis to produce citric acid; this reaction causes colorless DTNB to transform into yellow TNB, TNB has a characteristic absorbing value at 412nm.The activity of citrate synthase could be obtained by calculated the increase rate of the light absorption at 412nm.
Kit Components
Extraction Buffer
ReagentI
Reagent II
Reagent III
Reagent IV
Reagent V
Reagent VI
ReagentI
Reagent II
Reagent III
Reagent IV
Reagent V
Reagent VI
Usage Notes
deltaA of Test Tube should be between 0.01-0.3, the sample should be diluted further if deltaA of Test Tube >0.3.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract CS from fresh samples in order to ensure the enzyme activity.
Please take protective measures when operating, because ReagentII has certain toxicity.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract CS from fresh samples in order to ensure the enzyme activity.
Please take protective measures when operating, because ReagentII has certain toxicity.
Product Categories/Family for CS assay kit
Biochemical Assay Kit; Cell Metabolism; Cytology
Other Notes
Small volumes of CS assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CS purchase
MBS9719200 is an assay kit targeting CS. Also known as Citrate Synthase (CS) Activity Assay Kit (Colorimetric).