StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
Application Details
Detect Range:Request Information Sensitivity:Request Information Sample Type:Serum, Plasma, Other biological fluids Sample Volume: 1-200 μL Assay Time:1-4.5h Detection wavelength:450 nm
Detection Method:Competitive ELISA Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fever Virus Anitibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Fever Virus Anitibody and incubated. The competitive inhibition reaction is launched between with HRP labeled Fever Virus Anitibody and unlabeled Fever Virus Anitibody with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Fever Virus Anitibody in the sample. The color development is stopped and the intensity of the color is measured.
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