CBEL-ACEHIS3-5 | Custom Human/Mouse Acetylated-Histone H3 Cell-based

Product Description

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human, Mouse
Specificity	The antibody pair provided in this kit recognizes human/mouse Acetylated-Histone H3.
Accession Number	P84243\|P84244
Gene Id	3020\|3021
Gene Symbols	H3F3A, H3F3B, H3.3A, H3F3, PP781, H3.3B
Protein Name / Synonyms	Histone H3.3
Research Area	Post-Translational Modifications, Phosphorylation, Acetylation, Epigenetics
Shipping Type	Blue ice
Storage	-20°C

Rapidly measures acetylated protein in lysates
Simultaneously measure acetylated protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Kit Components

Other Materials Required

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Upon receipt, the kit should be stored at 20°C. Please use within 6 months from the date of shipment.

Custom Human/Mouse Acetylated-Histone H3 Cell-based ELISA Kit