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> Cy 5 amine (TFA)

Cy 5 amine (TFA)

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Cat# HY-D1321A-5mg

Size : 5mg

Brand : MedChemExpress

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Phone : +1 850 650 7790

Description

Cy 5 amine (Cyanine5 amine) TFA is a fluorescent dye. Cy 5 amine TFA can be used in the preparation of Cy5.5-labeled compound or polymers, which can be used for imaging cellular process and trafficking[1].

In Vitro

Cy5-amine TFA (0.1 mg/mL; 24 h protected from light) efficiently conjugates to activated Chondroitin sulfate (HY-B2162) to form fluorescently labeled Cy5-CS[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cy 5 amine TFA Related Antibodies
In Vivo

Cy5 amine TFA (Cy5-CS) (40 μg/kg; p.o.; single dose) results in significantly higher and longer-lasting whole-body fluorescence, extended intestinal retention with enhanced jejunal accumulation, and greater hepatic accumulation compared to free Cy5-amine in healthy male C57BL/6 mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

731.33

Formula

C40H54ClF3N4O3
Appearance

Solid

Color

Brown to dark brown

SMILES

O=C(CCCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C3=[N+](C)C4=C(C3(C)C)C=CC=C4)C=CC=C2)NCCCCCCN.OC(C(F)(F)F)=O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 15.38 mg/mL (21.03 mM; ultrasonic and warming and adjust pH to 9 with 1 M NaOH and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3674 mL 6.8369 mL 13.6737 mL
5 mM 0.2735 mL 1.3674 mL 2.7347 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References