Cytokeratin, Multi (Multiple)
Cat# 172084-100ug
Size : 100ug
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
172084Cytokeratin, Multi (Multiple)
Specifications
This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 0.5-1.0ug/ml
Western Blot: 0.5-1.0ug/ml
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1.0ug/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Positive Control:
A431 cells, Skin, Colon carcinoma
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.