DAB Chromogen Kit
Cat# MBS826735-5x60mL
Size : 5x60mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Immunohistochemical analysis staining in human breast carcinoma formalin fixed paraffin-embedded tissue section. The section was detected using DAB Chromogen Kit. The section was then counterstained with haematoxylin and mounted with Neutral Gum.
DAB Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS826735Background: 3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. This kit is prepared for Immunohistochemistry, Immunoblot… More details
Product Overview
Online Datasheet in PDFProduct Name
DAB, Reagent
Full Product Name
DAB Chromogen Kit
Preparation and Storage
All kit components are stable at 4°C. Each component is stable for up to 12 months.
Kit Components
Component: DAB Substrate
Volume: 30 ml x 2
Storage: 4°C
Component: DAB Chromogen
Volume: 1.2 ml x 1
Storage: 4°C
Component: Technical Manual
Volume: 1 Manual
Volume: 30 ml x 2
Storage: 4°C
Component: DAB Chromogen
Volume: 1.2 ml x 1
Storage: 4°C
Component: Technical Manual
Volume: 1 Manual
Procedure
1: Add 20 ul DAB Chromogen into 1 ml DAB Substrate, mixing vial shortly before incubation.
2: Apply 50 ul DAB Reagent to each slide, incubate about 3-5 minutes
3: When sufficient staining has been acheived rinse slides in PBS 3 times for 3 minutes each time.
2: Apply 50 ul DAB Reagent to each slide, incubate about 3-5 minutes
3: When sufficient staining has been acheived rinse slides in PBS 3 times for 3 minutes each time.
Applications Tested/Suitable for DAB reagent
Immunohistochemistry (IHC)
Other Notes
Small volumes of DAB reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DAB purchase
MBS826735 is a reagent targeting DAB. Also known as DAB Chromogen Kit.
Additional Details
NCBI/Uniprot data below describe general gene information for DAB. It may not necessarily be applicable to this product.
Protein Family
Protein disabled
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.