DBH siRNA (Rat)
Cat# MBS829846-30nmol
Size : 30nmol
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
DBH siRNA – Dopamine Beta-Hydroxylase (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829846MBS829846 is an siRNA targeting DBH. Also known as DBH siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit DBH expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
DBH, siRNA
Full Product Name
DBH siRNA (Rat)
Product Gene Name
DBH sirna
Product Synonym Names
Dopamine beta-hydroxylase; Dopamine beta-monooxygenase
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
DBH siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
69,875 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DBH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for DBH sirna
RNA Interference (RNAi)
Other Notes
Small volumes of DBH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for DBH. It may not necessarily be applicable to this product.
NCBI Official Full Name
dopamine beta-hydroxylase
NCBI Official Synonym Full Names
dopamine beta-hydroxylase (dopamine beta-monooxygenase)
NCBI Official Symbol
Dbh
NCBI Official Synonym Symbols
DOPBHY
UniProt Protein Name
Dopamine beta-hydroxylase
UniProt Synonym Protein Names
Dopamine beta-monooxygenase
Protein Family
Dopamine beta-hydroxylase
UniProt Gene Name
Dbh
UniProt Entry Name
DOPO_RAT
NCBI Protein Information
dopamine beta-hydroxylase
NCBI Summary for DBH
oxidoreductase that catalyzes the conversion of 3,4 dihydroxyphenethylamine, ascorbate, and O(2) to noradrenaline, dehydroascorbate, and H(2) in norephinephrine biosynthesis [RGD, Feb 2006]
UniProt Comments for DBH
DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.
Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.17.1; Motility/polarity/chemotaxis; Amino Acid Metabolism - tyrosine; Apoptosis; Cell cycle regulation
Cellular Component: cell soma; apical part of cell; axon; dendrite; cytoplasmic membrane-bound vesicle; cytoplasm; integral to membrane; terminal button; synapse
Molecular Function: copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
Biological Process: fear response; response to peptide hormone stimulus; dopamine catabolic process; catecholamine metabolic process; response to pain; locomotory behavior; leukocyte mediated immunity; behavioral response to ethanol; glucose homeostasis; response to organic cyclic substance; response to estradiol stimulus; norepinephrine biosynthetic process; homoiothermy; visual learning; response to iron ion; associative learning; response to drug; octopamine metabolic process; sensory perception of taste; maternal behavior; response to amphetamine; multicellular organismal aging; cytokine production; social behavior; memory; regulation of cell proliferation; response to ethanol; response to copper ion; response to ozone; positive regulation of vasoconstriction; blood vessel remodeling; leukocyte migration
Show more Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.17.1; Motility/polarity/chemotaxis; Amino Acid Metabolism - tyrosine; Apoptosis; Cell cycle regulation
Cellular Component: cell soma; apical part of cell; axon; dendrite; cytoplasmic membrane-bound vesicle; cytoplasm; integral to membrane; terminal button; synapse
Molecular Function: copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
Biological Process: fear response; response to peptide hormone stimulus; dopamine catabolic process; catecholamine metabolic process; response to pain; locomotory behavior; leukocyte mediated immunity; behavioral response to ethanol; glucose homeostasis; response to organic cyclic substance; response to estradiol stimulus; norepinephrine biosynthetic process; homoiothermy; visual learning; response to iron ion; associative learning; response to drug; octopamine metabolic process; sensory perception of taste; maternal behavior; response to amphetamine; multicellular organismal aging; cytokine production; social behavior; memory; regulation of cell proliferation; response to ethanol; response to copper ion; response to ozone; positive regulation of vasoconstriction; blood vessel remodeling; leukocyte migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with DBH sirna
| Reference Product | PubMed Publications |
|---|---|
| Pnmt sirna | >190 publications with DBH and Pnmt |
| Comt sirna | >79 publications with DBH and Comt |
| Ddc sirna | >37 publications with DBH and Ddc |
| Phox2a sirna | >33 publications with DBH and Phox2a |
| Phox2b sirna | >33 publications with DBH and Phox2b |
| Maoa sirna | >27 publications with DBH and Maoa |
| Maob sirna | >5 publications with DBH and Maob |
Pathways associated with DBH sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Amine-derived Hormones Pathway antibodies | Amine-derived Hormones Pathway Diagram |
| Biogenic Amine Synthesis Pathway antibodies | Biogenic Amine Synthesis Pathway Diagram |
| Catecholamine Biosynthesis Pathway antibodies | Catecholamine Biosynthesis Pathway Diagram |
| Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies | Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram |
| Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies | Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram |
| Catecholamine Synthesis Pathway antibodies | Catecholamine Synthesis Pathway Diagram |
| Metabolic Pathways antibodies | Metabolic Pathways Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Metabolism Of Amino Acids And Derivatives Pathway antibodies | Metabolism Of Amino Acids And Derivatives Pathway Diagram |
| Tyrosine Metabolism Pathway antibodies | Tyrosine Metabolism Pathway Diagram |
Diseases associated with DBH sirna
| Disease Name | Pubmed Publications |
|---|---|
| Mental Disorders Antibodies | >258 publications with DBH and Mental Disorders |
| Nervous System Diseases Antibodies | >220 publications with DBH and Nervous System Diseases |
| Hypertension Antibodies | >95 publications with DBH and Hypertension |
| Movement Disorders Antibodies | >61 publications with DBH and Movement Disorders |
| Hypotension Antibodies | >42 publications with DBH and Hypotension |
| Pheochromocytoma Antibodies | >40 publications with DBH and Pheochromocytoma |
| Depressive Disorder Antibodies | >39 publications with DBH and Depressive Disorder |
| Parkinsonian Disorders Antibodies | >30 publications with DBH and Parkinsonian Disorders |
| Bipolar Disorder Antibodies | >29 publications with DBH and Bipolar Disorder |
| Kidney Diseases Antibodies | >20 publications with DBH and Kidney Diseases |
Organs/Tissues associated with DBH sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Heart Antibodies | >154 publications with DBH and Heart |