Diethylstilbestrol ELISA Kit
Cat# OKAO00122
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Diethylstilbestrol ELISA Kit (OKAO00122) |
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Predicted Species Reactivity | General | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Diethylstilbestrol ELISA Kit (OKAO00122) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Diethylstilbestrol has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Diethylstilbestrol antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||
ELISA Kit Range | 0.025-2.025 ppb | ||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 56-53-1 PubChem: 448537 | ||||||||||||||||||||
:: | Molecular Formula: C18H20O2 | ||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | Feed, meat, liver (Chicken, duck, porcine meat, beef and mutton), shrimp, fish, urine | ||||||||||||||||||||
Sensitivity |
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Specificity |
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Assay Info | Assay Methodology: Competitive Inhibition ELISA |
Alias Symbols | DES, Diethylstilbestrol, Stilbestrol, 56-53-1, Stilboestrol, Distilbene, Stilboestroform |
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Protein Name | Diethylstilbestrol |
Description of Target | Diethylstilbestrol is a synthetic, nonsteroidal form of estrogen. A well-known teratogen and carcinogen, diethylstilbestrol inhibits the hypothalamic-pituitary-gonadal axis, thereby blocking the testicular synthesis of testosterone, lowering plasma testosterone, and inducing a chemical castration. |
Molecular Weight | 268.3502 g/mol |