This kit is used to convert unmethylated cytosine residues into uracil for gene methylation analysis in genomic DNA. Methylated cytosine residues (5-methylcytosine) remain unchanged. PCR primers and/or probes can be designed to distinguish methylated cytosines from unmethylated.
DNA Bisulfite Conversion Kit (Magnetic Bead Method)
DNA Bisulfite Conversion Kit (Magnetic Bead Method)
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Product Description
Specifications
| Size | 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | DNA |
| Research Area | Methylation, Epigenetics |
| Shipping Type | Blue ice |
| Storage / Stability | Store kit in a dry environment at 2 – 30°C for up to 24 months. Kit must be used within the validity period to ensure correct performance. |
Kit Components
- Transformation Reagent; 4 vials (1.5 ml each)
- Magnetic Bead Solution B; 1 vial (0.6 ml)
- Binding Buffer B; 1 vial (30 ml)
- Wash Buffer B; 2 bottles (30 ml each)
- Desulphonation Buffer; 1 bottle (20 ml)
- Elution Buffer A; 3 vials (1.5 ml each)
Other Materials Required
- Magnetic frame
- Tabletop Centrifuge
- Vortex Mixer
- Water bath or dry heat block
- Thermocycler
- Pipettes and sterile nuclease free pipette tips (barrier tips recommended)
- Nuclease free microfuge, conical, and PCR tubes