DNMT1 siRNA (Human)
Cat# MBS8223879-30nmol
Size : 30nmol
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
DNMT1 siRNA – DNA (Cytosine-5)-Methyltransferase 1 Isoform a (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8223879MBS8223879 is an siRNA targeting DNMT1. Also known as DNMT1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit DNMT1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
DNMT1, siRNA
Full Product Name
DNMT1 siRNA (Human)
Product Gene Name
DNMT1 sirna
Product Synonym Names
AIM; CXXC9; DNMT; DNA; cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
DNMT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
144,465 Da
OMIM
126375
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DNMT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for DNMT1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of DNMT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for DNMT1. It may not necessarily be applicable to this product.
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform a
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol
DNMT1
NCBI Official Synonym Symbols
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names
CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
Protein Family
DNA
UniProt Gene Name
DNMT1
UniProt Synonym Gene Names
AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
UniProt Entry Name
DNMT1_HUMAN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1
NCBI Summary for DNMT1
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Show more UniProt Comments for DNMT1
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription regulation; Methyltransferase, DNA; Methyltransferase; Cell development/differentiation; EC 2.1.1.37
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; transcription factor binding; DNA-methyltransferase activity
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; gene silencing; chromatin modification; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Show more Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription regulation; Methyltransferase, DNA; Methyltransferase; Cell development/differentiation; EC 2.1.1.37
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; transcription factor binding; DNA-methyltransferase activity
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; gene silencing; chromatin modification; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with DNMT1 sirna
| Reference Product | PubMed Publications |
|---|---|
| DNMT3A sirna | >566 publications with DNMT1 and DNMT3A |
| DNMT3B sirna | >552 publications with DNMT1 and DNMT3B |
| HDAC1 sirna | >116 publications with DNMT1 and HDAC1 |
| PCNA sirna | >66 publications with DNMT1 and PCNA |
| HDAC2 sirna | >38 publications with DNMT1 and HDAC2 |
| EZH2 sirna | >38 publications with DNMT1 and EZH2 |
| EHMT2 sirna | >10 publications with DNMT1 and EHMT2 |
| USP7 sirna | >7 publications with DNMT1 and USP7 |
| SMARCA5 sirna | >5 publications with DNMT1 and SMARCA5 |
Pathways associated with DNMT1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| DNA Methylation Pathway antibodies | DNA Methylation Pathway Diagram |
| Epigenetic Regulation Of Gene Expression Pathway antibodies | Epigenetic Regulation Of Gene Expression Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Metabolic Pathways antibodies | Metabolic Pathways Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
Diseases associated with DNMT1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Carcinoma Antibodies | >242 publications with DNMT1 and Carcinoma |
| Adenocarcinoma Antibodies | >109 publications with DNMT1 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >94 publications with DNMT1 and Breast Neoplasms |
| Liver Diseases Antibodies | >67 publications with DNMT1 and Liver Diseases |
| Cell Transformation, Neoplastic Antibodies | >66 publications with DNMT1 and Cell Transformation, Neoplastic |
| Disease Models, Animal Antibodies | >65 publications with DNMT1 and Disease Models, Animal |
| Carcinoma, Hepatocellular Antibodies | >63 publications with DNMT1 and Carcinoma, Hepatocellular |
| Lung Neoplasms Antibodies | >60 publications with DNMT1 and Lung Neoplasms |
| Liver Neoplasms Antibodies | >56 publications with DNMT1 and Liver Neoplasms |
| Neoplasms, Experimental Antibodies | >47 publications with DNMT1 and Neoplasms, Experimental |
Organs/Tissues associated with DNMT1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >195 publications with DNMT1 and Blood |
| Brain Antibodies | >148 publications with DNMT1 and Brain |
| Liver Antibodies | >129 publications with DNMT1 and Liver |
| Lung Antibodies | >105 publications with DNMT1 and Lung |
| Embryonic Tissue Antibodies | >51 publications with DNMT1 and Embryonic Tissue |
| Prostate Antibodies | >51 publications with DNMT1 and Prostate |
| Stomach Antibodies | >44 publications with DNMT1 and Stomach |
| Muscle Antibodies | >41 publications with DNMT1 and Muscle |
| Skin Antibodies | >38 publications with DNMT1 and Skin |
| Nerve Antibodies | >37 publications with DNMT1 and Nerve |