Dolphin Cytokine Array Q1 (Glass Slide)

Cat# QAD-CYT-1-4

Size : 50SampleKit

Brand : RayBiotech

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Phone : +1 850 650 7790

Dolphin Cytokine Array Q1

Quantibody® Dolphin Cytokine Array 1 Kit. Detects 10 Dolphin Cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesDolphin
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected10
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 101337436
  • Accession #: Q9TV67
IL-1 beta
(IL-1 F2)
IL-1 Ra
(IL-1 F3)
IL-2
  • Gene Symbol: IL2
  • Accession #: B3V280
IL-6
IL-8
(CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 101331085
  • Accession #: Q7YRB5
IL-13
IL-17A
IP-10
(CXCL10)
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 101318178
  • Accession #: Q9BEA1

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Dolphin Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAD-CYT-1.gal)
Excel (QAD-CYT-1_Analysis_Tool.xls)