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Reagents and instruments for immunology, cell biology and molecular biology.

Donkey IgG (H&L) Antibody Peroxidase Conjugated

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Cat# orb346730-20mg

Size : 20mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Biorbyt

Request more information

Contact local distributor :

Phone : +1 850 650 7790

Donkey IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb346730

This product can be custom made

Description

Donkey IgG (H&L) antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Donkey
Application Notes	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Donkey IgG whole molecule
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG, and Donkey Serum.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	10.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Rabbit anti-Donkey IgG HRP conjugated Antibody, Rabbit anti-Donkey IgG Peroxidase Conjugated antibody

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Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed [orb347953]
Applications:
ELISA, IHC, WB
Reactivity:
Sheep
Species/Host:
Donkey
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 1 mg
Sheep IgG (H&L) Antibody Peroxidase Conjugated [orb347959]
Applications:
ELISA, IHC, WB
Reactivity:
Sheep
Species/Host:
Donkey
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 2 mg
Donkey IgG (H&L) Antibody Peroxidase Conjugated [orb347994]
Applications:
ELISA, IHC, WB
Reactivity:
Donkey
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 2 mg

Protocol Information

WB

Western Blot (IB, immunoblot)

IHC

Immunohistochemistry

ELISA

Enzyme-linked Immunosorbent Assay (EIA)